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A common sequence motif involved in selection of transcription start sites of Arabidopsis and budding yeast tRNA genes

机译：参与拟南芥和芽生酵母tRNA基因转录起始位点选择的共同序列基序

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The transcription start site (TSS) is useful to predict gene and to understand transcription initiation. Although vast data on mRNA TSSs are available, little is known about tRNA genes because of rapid processing. Using a tobacco in vitro transcription system under conditions of impaired 5′ end processing, TSSs were determined for 64 Arabidopsis tRNA genes. This analysis revealed multiple TSSs distributed in a region from 10 to 2?bp upstream of the mature tRNA coding sequence (??10 to ??2). We also analyzed 31 Saccharomyces cerevisiae tRNA genes that showed a smaller number but a broader distribution (??13 to ??1) of TSSs. In both cases, transcription was initiated preferentially at adenosine, and a common ‘TCAACA’ sequence was found spanning the TSSs. In plant, this motif caused multiple TSSs to converge at one site and enhanced transcription. The TATA-like sequence upstream of Arabidopsis tRNA genes also contributed to TSS selection.
机译：转录起始位点（TSS）可用于预测基因和了解转录起始。尽管可以获得关于mRNA TSS的大量数据，但由于处理迅速，对tRNA基因了解甚少。在5'末端加工受损的条件下，使用烟草体外转录系统，确定了64个拟南芥tRNA基因的TSS。该分析揭示了多个TSS分布在成熟tRNA编码序列上游的10-2bp的区域（Δ10至Δ2 2）中。我们还分析了31个啤酒酵母tRNA基因，它们显示的TSS数量较少但分布较广（Δε13至Δε1）。在这两种情况下，转录都优先从腺苷开始，并且发现跨TSS的共有“ TCAACA”序列。在植物中，该基序导致多个TSS集中在一个位点并增强了转录。拟南芥tRNA基因上游的TATA样序列也有助于TSS的选择。

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