JBP1-seq: A fast and efficient method for genome-wide profiling of 5hmC

Libin Cui; Tzu Hung Chung; Darany Tan; Xueguang Sun; Xi-Yu Jia

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JBP1-seq: A fast and efficient method for genome-wide profiling of 5hmC

机译：JBP1-seq：一种快速高效的5hmC全基因组谱分析方法

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Wedevelopedanovelapproach,J-bindingprotein1sequencing(JBP1-seq),thatcombinesthebenefitsofanimprovedrecombinantJBP1protein,Nextera-basedlibraryconstruction,andnext-generationsequencing(NGS)forgenome-wideprofilingof5-hydroxymethylcytosine(5hmC).ComparedwiththeoriginalJBP1,thisnewrecombinantJBP1wasbiotinylatedinvivoandconjugatedtomagneticbeadsviabiotinndash;streptavidininteractions.Thesemodificationsallowedamoreefficientandconsistentpull-downofβ-glucosyl-5-hydroxymethylcytosine(β-glu-5hmC),andsequence-readylibrariescanbegeneratedwithin4.5#xA0;hfromDNAinputsaslowas50#xA0;ng.5hmCenrichmentofhumanbrainDNAusingthenewJBP1resultedinover25,000peakscalled,whichissignificantlyhigherthanthe4003peaksenrichedusingtheoldJBP1.Comparisonofthetechnicalduplicatesandvalidationswithotherplatformsindicatedtheresultsarereproducibleandreliable.Thus,JBP1-seqprovidesafast,efficient,andcost-effectivemethodforaccurate5hmCgenome-wideprofiling./p/div

机译：Wedevelopedanovelapproach，J-bindingprotein1sequencing（JBP1-SEQ），thatcombinesthebenefitsofanimprovedrecombinantJBP1protein，NEXTERA-basedlibraryconstruction，andnext-generationsequencing（NGS）forgenome-wideprofilingof5羟甲基（5hmC的）.ComparedwiththeoriginalJBP1，thisnewrecombinantJBP1wasbiotinylatedinvivoandconjugatedtomagneticbeadsviabiotinndash; streptavidininteractions.Thesemodificationsallowedamoreefficientandconsistentpull-downofβ葡糖基5-羟甲基（β- GLU-5hmC的），andsequence-readylibrariescanbegeneratedwithin4.5＃XA0; hfromDNAinputsaslowas50＃XA0; ng.5hmCenrichmentofhumanbrainDNAusingthenewJBP1resultedinover25,000peakscalled，whichissignificantlyhigherthanthe4003peaksenrichedusingtheoldJBP1.Comparisonofthetechnicalduplicatesandvalidationswithotherplatformsindicatedtheresultsarereproducibleandreliable.Thus，JBP1-seqprovidesafast，高效，andcost-effectivemethodforaccurate5hmCgenome-wideprofiling

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《Genomics》 |2014年第5期|共页
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Libin Cui; Tzu Hung Chung; Darany Tan; Xueguang Sun; Xi-Yu Jia;
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中图分类医学遗传学;
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1. JBP1-seq: A fast and efficient method for genome-wide profiling of 5hmC [J] . Cui Libin, Chung Tzu Hung, Tan Darany, Genomics . 2014,第5期

机译：JBP1-seq：一种快速高效的5hmC全基因组谱分析方法
2. A Highly Sensitive and Robust Method for Genome-wide 5hmC Profiling of Rare Cell Populations [J] . Han Dali, Lu Xingyu, Shih Alan H., Molecular cell . 2016,第4期

机译：全基因组5hmC稀有细胞群体的高灵敏度和鲁棒性分析方法。
3. Fast and accurate methods for the performance testing of highly-efficient c-Si photovoltaic modules using a 10 ms single-pulse solar simulator and customized voltage profiles [J] . A Virtuani, G Rigamonti, G Friesen, Measurement Science & Technology . 2012,第11期

机译：使用10 ms单脉冲太阳能模拟器和自定义电压曲线的快速，准确的方法，用于高效c-Si光伏模块的性能测试
4. Efficient and Fast Analysis for Detecting High Order Gene-by-Gene Interactions in a Genome-Wide Association Study [C] . Oh Sohee, Lee Jaehoon, Kwon Min-Seok, 2011 IEEE International Conference on Bioinformatics and Biomedicine . 2011

机译：在全基因组关联研究中检测高阶基因间相互作用的高效快速分析
5. Computing the Microbiome: Faster, More Accurate and More Efficient Methods for the Analysis of Metagenomes. [D] . Ounit, Rachid. 2017

机译：计算微生物组：更快，更准确和更高效的分析基因组的方法。
6. A Highly Sensitive and Robust Method for Genome-wide 5hmC Profiling of Rare Cell Populations [O] . Dali Han, Xingyu Lu, Alan H. Shih, -1

机译：全基因组5hmC稀有细胞群体的高灵敏度和鲁棒性分析。
7. JBP1-seq: A fast and efficient method for genome-wide profiling of 5hmC [O] . Cui Libin, Chung Tzu Hung, Tan Darany, 2014

机译：JBP1-seq：一种快速高效的5hmC全基因组谱分析方法

JBP1-seq: A fast and efficient method for genome-wide profiling of 5hmC

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