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首页> 外文期刊>Genomics >Cloning and identification of two novel NBCe1 splice variants from mouse reproductive tract tissues: A comparative study of NCBT genes
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Cloning and identification of two novel NBCe1 splice variants from mouse reproductive tract tissues: A comparative study of NCBT genes

机译：小鼠生殖道组织中两个新型NBCe1剪接变体的克隆与鉴定：NCBT基因的比较研究

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摘要

Na+-coupled HCO3? transporters (NCBTs) of the SLC4 family play critical roles in pH regulation as well as transepithelial HCO3? transport. We systematically examined, in the mouse reproductive tract tissues, the mRNA expression of five NCBTs as well as the five NBCe1 (Slc4a4) variants NBCe1-A through -E, of which NBCe1-D and NBCe1-E are novel. Cloning of NBCe1-D and NBCe1-E, both lacking a 27-nucleotide cassette I, reveals a novel alternative splicing unit in the mouse Slc4a4 gene. Transcripts of Slc4a4 lacking cassette I are expressed in diverse murine tissues as shown by RT-PCR analysis and in diverse tissues of other vertebrate species as shown by blast against GenBank database. Genomic sequence analysis indicates that cassette I of SLC4A4 is conserved in all NCBT genes except for SLC4A5, which presumably lost cassette I during its evolution. Our present study represents an important step towards understanding the molecular physiology of NBCe1, and presumably other NCBTs.
机译：Na +耦合的HCO3？ SLC4家族的转运蛋白（NCBT）在pH调节以及跨上皮HCO3中起着关键作用？运输。我们系统地检查了小鼠生殖道组织中，五个NCBTs以及五个NBCe1（Slc4a4）变体NBCe1-A至-E的mRNA表达，其中NBCe1-D和NBCe1-E是新颖的。都缺少27个核苷酸的I盒的NBCe1-D和NBCe1-E的克隆揭示了小鼠Slc4a4基因中新的可变剪接单元。缺少盒I的Slc4a4转录本在RT-PCR分析显示的多种鼠类组织中表达，而针对GenBank数据库的blast显示在其他脊椎动物物种的多种组织中表达。基因组序列分析表明，除SLC4A5外，SLC4A4的盒I在所有NCBT基因中都是保守的，SLC4A5可能在进化过程中丢失了盒I。我们目前的研究代表了迈向了解NBCe1以及大概其他NCBT分子生理学的重要一步。

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