Transcript profiling of microRNAs during the early development of the maize brace root via Solexa sequencing

Peng Liu; Kang Yan; Yun-xue Lei; Rui Xu; Yue-min Zhang; Guo-dong Yang; Jin-guang Huang; Chang-Ai Wu; Cheng-Chao Zheng

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Transcript profiling of microRNAs during the early development of the maize brace root via Solexa sequencing

机译：通过Solexa测序在玉米支架根的早期发育过程中对microRNA进行转录分析

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TocharacterizethemicroRNAsthatcontributetothedevelopmentofbraceroot,Solexahigh-throughputsequencingofthreelibrariesderivedfromtissuesofnode(N),nodeswithjust-emergedbraceroots(NR),andnodeswithjust-emergedbracerootsafterIAAtreatment(NRI)wasperformed.Total650,793,957,303and1,082,948genome-matcheduniquereadswereobtainedinN,NRandNRIlibraries,respectively.Furtheranalysisconfirmedtheauthenticityof137knownmiRNAsandthediscoveryof159novelmiRNAsinmaize.14conservedand16novelmiRNAsdifferentiallyexpressedinbraceroot,aswellas15targetgenes,wereidentifiedandvalidatedbyqRT-PCRduringmaizebracerootdevelopment.Moreover,weidentified9miRNAprecursor-matchednovelsRNAsthatmayformmiRNAclusters,aswellas24#xA0;ntsiRNAsinthethreelibraries.Inaddition,wesuggestthatauxinrepresentaregulatorinbracerootdevelopmentandcanberegulatedattheposttranscriptionallevelbymiRNAs./p/div

机译：TocharacterizethemicroRNAsthatcontributetothedevelopmentofbraceroot，Solexahigh-throughputsequencingofthreelibrariesderivedfromtissuesofnode（N），nodeswithjust-emergedbraceroots（NR），andnodeswithjust-emergedbracerootsafterIAAtreatment（NRI）wasperformed.Total650,793,957,303and1,082,948genome-matcheduniquereadswereobtainedinN，NRandNRIlibraries，respectively.Furtheranalysisconfirmedtheauthenticityof137knownmiRNAsandthediscoveryof159novelmiRNAsinmaize.14conservedand16novelmiRNAsdifferentiallyexpressedinbraceroot，aswellas15targetgenes，wereidentifiedandvalidatedbyqRT-PCRduringmaizebracerootdevelopment.Moreover，我们确定了9个与miRNA前体相配的新颖RNA，它们可能在分子簇中形成miRNA簇，以及24＃xA0; ntsiRNA。另外，我们建议该生长素代表了调控子在根部发育中的作用，并且可以在转录后的水平上由miRNA进行调节。

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《Genomics》 |2013年第2期|共页
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Peng Liu; Kang Yan; Yun-xue Lei; Rui Xu; Yue-min Zhang; Guo-dong Yang; Jin-guang Huang; Chang-Ai Wu; Cheng-Chao Zheng;
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中图分类医学遗传学;
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1. Transcript profiling of microRNAs during the early development of the maize brace root via Solexa sequencing [J] . Peng Liu, Kang Yan, Yun-xue Lei, Genomics . 2013,第2期

机译：通过Solexa测序在玉米支架根的早期发育过程中对microRNA进行转录分析
2. Transcript profiling during the early development of the maize brace root via Solexa sequencing [J] . Li Yan-Jie, Fu Ya-Ru, Huang Jin-Guang, The FEBS journal . 2011,第1期

机译：通过Solexa测序在玉米支架根早期发育过程中进行转录谱分析
3. Transcriptome-Wide Comparative Analysis of microRNA Profiles in the Telogen Skins of Liaoning Cashmere Goats (Capra hircus) and Fine-Wool Sheep (Ovis aries) by Solexa Deep Sequencing [J] . DNA and Cell Biology . 2016,第11期

机译：通过Solexa深度测序对辽宁绒山羊（Capra hircus）和细毛羊（Ovis aries）的毛发生长期的microRNA谱进行转录组比较分析
4. Maize EST Sequencing and cDNA Microarray-Based Analysis of Transcript Profiles between Leaf and Root under Water-Deficit and Rewatering [C] . H.F.Lu, Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering at Guangxi University, J.L.Tang, 第二届植物分子育种国际会议论文集 . 2007

机译：缺水和补水条件下玉米EST序列和基于cDNA微阵列的转录本分析
5. Applying Mechanical Testing Strategies to Maize Brace Roots [D] . Erndwein, Lindsay. 2021

机译：将机械测试策略应用于玉米支架根
6. ZmRAP2.7 an AP2 Transcription Factor Is Involved in Maize Brace Roots Development [O] . Jieping Li, Fanjun Chen, Yanqing Li, -1

机译：ZmRAP2.7AP2转录因子参与玉米支撑根的发育
7. Transcript profiling of microRNAs during the early development of the maize brace root via Solexa sequencing [O] . Liu Peng, Yan Kang, Lei Yun-xue, 2013

机译：通过Solexa测序在玉米支架根早期发育过程中对microRNA进行转录分析

Transcript profiling of microRNAs during the early development of the maize brace root via Solexa sequencing

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