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Transcript profiling of microRNAs during the early development of the maize brace root via Solexa sequencing

机译：通过Solexa测序在玉米支架根的早期发育过程中对microRNA进行转录分析

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To characterize the microRNAs that contribute to the development of brace root, Solexa high-throughput sequencing of three libraries derived from tissues of node (N), nodes with just-emerged brace roots (NR), and nodes with just-emerged brace roots after IAA treatment (NRJ) was performed. Total 650,793, 957,303 and 1,082,948 genome-matched unique reads were obtained in N, NR and NRI libraries, respectively. Further analysis confirmed the authenticity of 137 known miRNAs and the discovery of 159 novel miRNAs in maize. 14 conserved and 16 novel miRNAs differentially expressed in brace root, as well as 15 target genes, were identified and validated by qRT-PCR during maize brace root development. Moreover, we identified 9 miRNA precursor-matched novel sRNAs that may form miRNA clusters, as well as 24 nt siRNAs in the three libraries. In addition, we suggest that auxin represent a regulator in brace root development and can be regulated at the posttranscriptional level by miRNAs.
机译：为了表征有助于牙根发育的microRNA，Solexa高通量测序了来自结点（N），具有刚出现的牙根（NR）的节点和具有刚出现的牙根的节点的三个库进行了IAA治疗（NRJ）。在N，NR和NRI文库中分别获得了650,793、957,303和1,082,948个基因组匹配的独特读物。进一步的分析证实了玉米中137种已知miRNA的真实性，并发现了159种新型miRNA。通过qRT-PCR鉴定并验证了玉米大括号根发育过程中在大括号根中差异表达的14个保守和16个新的miRNA，以及15个靶基因。此外，我们在这三个文库中鉴定了9种可能形成miRNA簇的miRNA前体匹配新型sRNA，以及24 nt siRNA。此外，我们建议生长素在支架根发育中代表调节剂，并且可以在转录后水平受miRNA调节。

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