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首页> 外文期刊>Genes & Genetic Systems >Individual chromosome identification, chromosomal collinearity and genetic-physical integrated map in Gossypium darwinii and four D genome cotton species revealed by BAC-FISH
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Individual chromosome identification, chromosomal collinearity and genetic-physical integrated map in Gossypium darwinii and four D genome cotton species revealed by BAC-FISH

机译：BAC-FISH揭示棉和4个D基因棉种的个体染色体鉴定，染色体共线性和遗传-物理整合图

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References(47) Cited-By(5) The study was conducted on the individual chromosome identification in Gossypium darwinii (AdDd), G. klotzschianum (D3k), G. davidsonii (D3d), G. armourianum (D2-1) and G. aridum (D4) using a multi-probe fluorescence of in situ hybridization (FISH) system. Comparative analysis on their genetic maps with that of physical maps was made as well. The FISH probes used contained two sets of bacterial artificial chromosome (BAC) clones [one is specific to 26 individual chromosomes from A and D subgenomes of G. hirsutum (Ah and Dh) while the other is a D genome centromere-specific BAC clone 150D24], 45S and 5S rDNA clones. The results showed that all Ad chromosomes were marked with the 13 Ah chromosome-specific BAC clones, whilst all Dd, D3k, D3d, D2-1 and D4 chromosomes and chromosomal arms were identified with the 13 Dh chromosome-specific BAC clones and the D genome centromere-specific BAC. According to the homology within D subgenomes which are between A (D) genome and A (D) subgenome, the systematic nomenclature for individual chromosome in the five species was established. The chromosomes of A (D) subgenomes in G. darwinii were named as Ad01-Ad13 (Dd01-Dd13). The chromosomes in D3k, D3d, D2-1 and D4 were named as D3k01-D3k13, D3d01-D3d13, D2-101-D2-113 and D401-D413, respectively. Based on the successful identification for individual chromosomes, 45S and 5S rDNA were located to the special chromosomes and chromosomal arms for all five species. And there appeared chromosomal collinearity from the BAC clones among different species by comparing BACs positions, which suggested that the majority of chromosome segment homology may exist between D genomes and D subgenome. Moreover, as the genetic map and physical map were integrated, the orientations of genetic maps for Dd and D genomes of diploid cotton were established. The orientations of some of chromosomes in genetic maps (Dd03, Dd04, Dd06, Dd09, Dd10 and Dd12) were found switched. The SSR marker in the middle of linkage group 04 was corrected at nearby the end of chromosome 04 by FISH. The study will be helpful to establish a theoretical basis using the wild gene bank to exploit more genes aiming for cotton breeding and will provide powerful evidences both for the evolution of Gossypium and assembling the sequences of the obtained and as well the on-going whole genome sequencing projects of cotton.
机译：参考文献（47）被引用的文献（5）进行了关于棉球菌（AdDd），克氏茨克氏菌（D3k），大卫氏芽孢杆菌（D3d），Armourianum（D2-1）和G的个体染色体鉴定的研究使用原位杂交（FISH）系统的多探针荧光技术检测。还对它们的遗传图谱与物理图谱进行了比较分析。使用的FISH探针包含两组细菌人工染色体（BAC）克隆[一组对G. hirsutum（Ah和Dh）的A和D亚基因组的26条个体染色体具有特异性，而另一组是D基因组着丝粒特异性BAC克隆150D24 ]，45S和5S rDNA克隆。结果显示，所有Ad染色体均标记有13 Ah染色体特异性BAC克隆，而所有Dd，D3k，D3d，D2-1和D4染色体和染色体臂均由13 Dh染色体特异性BAC克隆和D鉴定。基因组着丝粒特异性BAC。根据A（D）基因组和A（D）亚基因组之间的D亚基因组内的同源性，建立了五个物种中单个染色体的系统命名法。达尔文氏菌中A（D）亚基因组的染色体命名为Ad01-Ad13（Dd01-Dd13）。 D3k，D3d，D2-1和D4中的染色体分别命名为D3k01-D3k13，D3d01-D3d13，D2-101-D2-113和D401-D413。在成功鉴定单个染色体的基础上，将45S和5S rDNA定位于所有五个物种的特殊染色体和染色体臂上。通过比较BACs的位置，不同物种之间的BAC克隆出现了染色体共线性，这表明D基因组和D亚基因组之间可能存在大部分染色体区段同源性。此外，通过整合遗传图谱和物理图谱，建立了二倍体棉Dd和D基因组遗传图谱的方向。发现遗传图谱中某些染色体的方向（Dd03，Dd04，Dd06，Dd09，Dd10和Dd12）已切换。通过FISH校正了04号连锁群中间的SSR标记。该研究将为利用野生基因库开发更多的棉花育种基因奠定理论基础，并将为棉属的进化，组装所得序列以及正在进行的整个基因组提供有力的证据。棉花测序项目。

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