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首页> 外文期刊>Genomics >The DNA-binding activity of an AP2 protein is involved in transcriptional regulation of a stress-responsive gene, SiWD40, in foxtail millet
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The DNA-binding activity of an AP2 protein is involved in transcriptional regulation of a stress-responsive gene, SiWD40, in foxtail millet

机译：AP2蛋白的DNA结合活性与谷子中应激反应基因SiWD40的转录调控有关。

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A differentially expressed transcript, encoding a putative WD protein (Setaria italica WD40; SiWD40), was identified in foxtail millet. Tertiary structure modeling revealed that its C-terminus possesses eight blade β-propeller architecture. Its N-terminal has three α-helices and two 310-helices and was highly induced by different abiotic stresses. The SiWD40:GFP fusion protein was nuclear localized. Promoter analysis showed the presence of many cis-acting elements, including two dehydration responsive elements (DRE). A stress-responsive SiAP2 domain containing protein could specifically bind to these elements in the SiWD40 promoter. Thus, for the first time, we report that DREs probably regulate expression of SiWD40 during environmental stress. Molecular docking analysis revealed that the circumference of the β‐propeller structure was involved in an interaction with a SiCullin4 protein, supporting the adaptability of SiWD40 to act as a scaffold. Our study thus provides a vital clue for near future research on the stress-regulation of WD proteins.
机译：在谷子中鉴定出差异表达的转录本，该转录本编码一种假定的WD蛋白（Setaria italica WD40; SiWD40）。三级结构建模表明，其C端拥有八个叶片β螺旋桨架构。它的N末端有3个α螺旋和2个310螺旋，并且受到不同的非生物胁迫的强烈诱导。 SiWD40：GFP融合蛋白是核定位的。启动子分析表明存在许多顺式作用元件，包括两个脱水反应元件（DRE）。含有应激反应的SiAP2结构域的蛋白质可以特异性地与SiWD40启动子中的这些元件结合。因此，我们首次报道了DRE可能在环境胁迫下调节SiWD40的表达。分子对接分析表明，β-螺旋桨结构的周缘参与了与SiCullin4蛋白的相互作用，从而支持了SiWD40作为支架的适应性。因此，我们的研究为WD蛋白的应激调控的近期研究提供了重要的线索。

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