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X-ray photon correlation spectroscopy of protein dynamics at nearly diffraction-limited storage rings

机译：X射线光子相关光谱在接近衍射极限的存储环处的蛋白质动力学

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This study explores the possibility of measuring the dynamics of proteins in solution using X-ray photon correlation spectroscopy (XPCS) at nearly diffraction-limited storage rings (DLSRs). We calculate the signal-to-noise ratio (SNR) of XPCS experiments from a concentrated lysozyme solution at the length scale of the hydrodynamic radius of the protein molecule. We take into account limitations given by the critical X-ray dose and find expressions for the SNR as a function of beam size, sample-to-detector distance and photon energy. Specifically, we show that the combined increase in coherent flux and coherence lengths at the DLSR PETRA IV yields an increase in SNR of more than one order of magnitude. The resulting SNR values indicate that XPCS experiments of biological macromolecules on nanometre length scales will become feasible with the advent of a new generation of synchrotron sources. Our findings provide valuable input for the design and construction of future XPCS beamlines at DLSRs.
机译：这项研究探索了使用X射线光子相关光谱（XPCS）在接近衍射极限的存储环（DLSR）上测量溶液中蛋白质动力学的可能性。我们从浓缩的溶菌酶溶液在蛋白质分子的流体动力学半径的长度尺度上计算XPCS实验的信噪比（SNR）。我们考虑了临界X射线剂量所带来的限制，并找到了SNR的表达式，该表达式与光束大小，样品到检测器的距离和光子能量有关。具体而言，我们表明在DLSR PETRA IV处相干通量和相干长度的组合增加会导致SNR的增加超过一个数量级。所得的SNR值表明，随着新一代同步加速器源的出现，纳米级生物大分子的XPCS实验将变得可行。我们的发现为DLSR上未来XPCS光束线的设计和施工提供了宝贵的意见。

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