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首页> 外文期刊>Genetics research international >Characterization andIn SilicoAnalysis of Pregnancy-Associated Glycoprotein-1 Gene of Buffalo(Bubalus bubalis)
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Characterization andIn SilicoAnalysis of Pregnancy-Associated Glycoprotein-1 Gene of Buffalo(Bubalus bubalis)

机译：水牛（Bubalus bubalis）妊娠相关糖蛋白-1基因的表征及计算机分析

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Pregnancy-Associated Glycoproteins (PAGs) are trophoblastic proteins belonging to the Aspartic proteinase family secreted by different placental cells of many mammalian species. They play a pivotal role in placentogenesis, foetomaternal unit remodeling, and implantation. The identification of the genes encoding those proteins will be helpful to unravel the intricate embryogenomic functions during pregnancy establishment. Considering importance of these proteins, the present study was undertaken to characterize the pregnancy associated glycoprotein-1 gene of buffalo. An 1181 base pairs buffalo Pregnancy-Associated Glycoprotein PAG-1 gene was PCR amplified from the RNA obtained from the fetal cotyledons. BLAST analysis of the buffalo PAG-1 sequence retrieved a total of 20 cattle, 5 goat, and 4 sheep PAG sequences, exhibiting more than 80% similarity. Buffalo PAG-1 gene contained an uninterrupted open reading frame of 1140 base pairs encoding 380 amino acids that possess a 15 amino acid signal peptide and mature peptide of 365 amino acids. The phylogenetic study of the buffalo PAG-1 gene revealed buffalo PAG-1 is more related to cattle, goat, and sheep PAG-1 sequences. By this study characterization of buffalo PAG-1 gene and its evolutionary relationship was deduced for the first time.
机译：妊娠相关糖蛋白（PAG）是滋养层蛋白，属于天冬氨酸蛋白酶家族，由许多哺乳动物的不同胎盘细胞分泌。它们在胎盘发生，胎儿母体单位重塑和植入中起关键作用。编码这些蛋白质的基因的鉴定将有助于阐明妊娠建立过程中复杂的胚胎基因组功能。考虑到这些蛋白质的重要​​性，本研究旨在表征与水牛有关的妊娠糖蛋白-1基因。从胎儿子叶获得的RNA中PCR扩增出1181个碱基对的水牛妊娠相关糖蛋白PAG-1基因。 Buffalo PAG-1序列的BLAST分析共检索到20头牛，5只山羊和4只绵羊PAG序列，表现出超过80％的相似性。布法罗PAG-1基因包含1140个碱基对的不间断开放阅读框，其编码380个氨基酸，具有15个氨基酸的信号肽和365个氨基酸的成熟肽。对水牛PAG-1基因的系统发育研究表明，水牛PAG-1与牛，山羊和绵羊PAG-1序列更相关。通过这项研究，首次推导了水牛PAG-1基因的特征及其进化关系。

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