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首页> 外文期刊>Journal of Molecular Genetics >Characterization and in silico Analysis of Pregnancy Associated Glycoprotein-1 Gene of Buffalo (Bubalus bubalis)
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Characterization and in silico Analysis of Pregnancy Associated Glycoprotein-1 Gene of Buffalo (Bubalus bubalis)

机译：水牛（Bubalus bubalis）妊娠相关糖蛋白-1基因的表征和计算机分析

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Pregnancy Associated Glycoproteins are trophoblastic proteins belonging to the Aspartic proteinase family secreted by different mammalian species. They play a pivotal role in placentogenesis, foeto-maternal unit remodeling and implantation. The identification of the genes encoding those proteins will be helpful to unravel the intricate embryogenomic functions during pregnancy establishment. Considering importance of these proteins, the present study was undertaken to characterize the pregnancy associated glycoprotein-1 gene of buffalo. An 1181 base pairs buffalo Pregnancy Associated Glycoprotein PAG- 1 gene was PCR amplified from the RNA obtained from the fetal cotyledons. BLAST analysis of the buffalo PAG- 1 sequence retrieved a total of 94 sequences which includes 35 cattle, 5 goat and 4 sheep PAG sequences, exhibiting more than 80% similarity. In silico analysis of buffalo PAG-1 gene revealed an uninterrupted open reading frame of 1140 base pairs encoding 380 amino acids possessing 15 amino acid signal peptide and mature peptide of 365 amino acids which is proteolytically inactive due to key mutations. The potential sites for glycosylation were also deduced. The phylogenetic study of the buffalo PAG-1 gene revealed buffalo PAG-1 is more related to cattle, goat and sheep PAG-1 sequences. Characterization and phylogenetic analysis of buffalo PAG-1 gene revealed many conserved regions and its proteolytic inactivity.
机译：妊娠相关糖蛋白是属于不同哺乳动物物种分泌的天冬氨酸蛋白酶家族的滋养层蛋白。它们在胎盘发生，胎儿母体单位重塑和植入中起关键作用。编码这些蛋白质的基因的鉴定将有助于阐明妊娠建立过程中复杂的胚胎基因组功能。考虑到这些蛋白质的重要​​性，本研究旨在表征与水牛有关的妊娠糖蛋白-1基因。从获自胎儿子叶的RNA中PCR扩增出1181个碱基对的水牛妊娠相关糖蛋白PAG-1基因。 Buffalo PAG-1序列的BLAST分析检索到总共94个序列，其中包括35头牛，5个山羊和4个绵羊PAG序列，表现出80％以上的相似性。在对水牛PAG-1基因的计算机分析中，发现不间断的1140个碱基对的开放阅读框，该碱基对编码380个氨基酸，具有15个氨基酸信号肽和365个氨基酸的成熟肽，由于关键突变而蛋白水解性失活。还推导了糖基化的潜在位点。对水牛PAG-1基因的系统发育研究表明，水牛PAG-1与牛，山羊和绵羊PAG-1序列更相关。水牛PAG-1基因的表征和系统发育分析表明，许多保守区及其蛋白水解失活。

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