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首页> 外文期刊>Genes and environment >Detection of Genotoxic Nucleosides, 8-Hydroxydeoxyguanosine, 8-Hydroxyguanosine and Free Base 8-Hydroxyguanine, in Fish Food Products
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Detection of Genotoxic Nucleosides, 8-Hydroxydeoxyguanosine, 8-Hydroxyguanosine and Free Base 8-Hydroxyguanine, in Fish Food Products

机译：鱼食品中遗传毒性核苷，8-羟基脱氧鸟嘌呤，8-羟基鸟嘌呤和游离碱8-羟基鸟嘌呤的检测

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References(11) Cited-By(2) 8-Hydroxydeoxyguanosine (8-OH-dG) is a DNA base modification induced by reactive oxygen species (ROS), and is analyzed in cellular DNA and urine as a marker of oxidative stress. We now report that 8-OH-dG, the ribonucleoside 8-OH-Guo and the free base 8-OH-Gua were detected in fish food products, such as salted dried sardines (Maruboshi) and dried small fishes (Iriko and Shirasu), which are often consumed in Japan. Water extracts of these fish products were analyzed by an HPLC-ECD method, using anion exchange- and reverse phase-columns. Various amounts of 8-OH-Gua (25 ng-22 μg/g), 8-OH-Guo (28-406 ng/g) and 8-OH-dG (5-60 ng/g) were detected in these foods. The amounts of 8-OH-dG and 8-OH-Guo increased upon heating or broiling of these fish products. This is the first report on the presence of genotoxic nucleoside analogues in food. These results provide the warnings that these nucleosides analogues might be food carcinogens, because they are incorporated into DNA by a salvage pathway and show genotoxicity.
机译：参考文献（11）Cited-By（2）8-Hydroxydeoxyguanosine（8-OH-dG）是一种由活性氧（ROS）诱导的DNA碱基修饰，并在细胞DNA和尿液中进行分析，作为氧化应激的标志物。我们现在报告在鱼食品中检测到8-OH-dG，核糖核苷8-OH-Guo和游离碱8-OH-Gua，例如盐腌的沙丁鱼干（Maruboshi）和干小鱼（Iriko和Shirasu） ，通常在日本消费。通过HPLC-ECD方法，使用阴离子交换和反相色谱柱分析了这些鱼产品的水提取物。在这些食物中检测到各种量的8-OH-Gua（25 ng-22μg/ g），8-OH-Guo（28-406 ng / g）和8-OH-dG（5-60 ng / g） 。通过加热或烘烤这些鱼产品，增加了8-OH-dG和8-OH-Guo的含量。这是有关食物中存在遗传毒性核苷类似物的第一份报告。这些结果警告说这些核苷类似物可能是食物致癌物，因为它们通过挽救途径掺入DNA中并显示出遗传毒性。

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