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首页> 外文期刊>IUCrJ >Rapid sample delivery for megahertz serial crystallography at X-ray FELs
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Rapid sample delivery for megahertz serial crystallography at X-ray FELs

机译：在X射线FEL上以兆赫兹级连续晶体学快速交付样品

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Liquid microjets are a common means of delivering protein crystals to the focus of X-ray free-electron lasers (FELs) for serial femtosecond crystallography measurements. The high X-ray intensity in the focus initiates an explosion of the microjet and sample. With the advent of X-ray FELs with megahertz rates, the typical velocities of these jets must be increased significantly in order to replenish the damaged material in time for the subsequent measurement with the next X-ray pulse. This work reports the results of a megahertz serial diffraction experiment at the FLASH FEL facility using 4.3 nm radiation. The operation of gas-dynamic nozzles that produce liquid microjets with velocities greater than 80 m s−1 was demonstrated. Furthermore, this article provides optical images of X-ray-induced explosions together with Bragg diffraction from protein microcrystals exposed to trains of X-ray pulses repeating at rates of up to 4.5 MHz. The results indicate the feasibility for megahertz serial crystallography measurements with hard X-rays and give guidance for the design of such experiments.
机译：液体微射流是将蛋白质晶体传递到X射线自由电子激光（FEL）焦点以进行连续飞秒晶体学测量的一种常用方法。焦点处的高X射线强度引发了微射流和样品的爆炸。随着兆赫兹速率的X射线FEL的出现，必须显着提高这些射流的典型速度，以便及时补充受损的材料，以便随后的下一个X射线脉冲进行测量。这项工作报告了在FLASH FEL设施中使用4.3 nm辐射进行兆赫兹系列衍射实验的结果。演示了可产生速度大于80 gasm s-1的液体微喷的气动喷嘴的操作。此外，本文提供了X射线诱发的爆炸的光学图像，以及暴露于X射线脉冲序列的蛋白质微晶的布拉格衍射，其重复频率高达4.5ingMHz。结果表明用硬X射线进行兆赫兹串行晶体学测量的可行性，并为此类实验的设计提供指导。

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