The Conformational Flexibility of the Acyltransferase from the Disorazole Polyketide Synthase Is Revealed by an X-ray Free-Electron Laser Using a Room-Temperature Sample Delivery Method for Serial Crystallography

Mathews Irimpan I.; Allison Kim; Robbins Thomas; Lyubimov Artem Y.; Uervirojnangkoorn Monarin; Brunger Axel T.; Khosla Chaitan; DeMirci Hasan; McPhillips Scott E.; Hollenbeck Michael; Soltis Michael; Cohen Aina E.

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The Conformational Flexibility of the Acyltransferase from the Disorazole Polyketide Synthase Is Revealed by an X-ray Free-Electron Laser Using a Room-Temperature Sample Delivery Method for Serial Crystallography

机译：通过使用用于连续晶体学的室温样品递送方法，通过X射线自由电子激光器揭示来自散唑聚酮合成酶的酰基转移酶的构象柔韧性

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The crystal structure of the trans-acyltransferase (AT) from the disorazole polyketide synthase (PKS) was determined at room temperature to a resolution of 2.5 angstrom using a new method for the direct delivery of the sample into an X-ray free-electron laser. A novel sample extractor efficiently delivered limited quantities of micro crystals directly from the native crystallization solution into the X-ray beam at room temperature. The AT structure revealed important catalytic features of this core PKS enzyme, including the occurrence of conformational changes around the active site. The implications of these conformational changes for polyketide synthase reaction dynamics are discussed.

机译：使用新方法在室温下在室温下在室温下测定来自脱唑聚酮化合酶（PKS）的反式酰基转移酶（AT）的晶体结构，以将样品直接递送到X射线自由电子激光器中的新方法。一种新的样品提取器，将直接从天然结晶溶液直接从天然结晶溶液中的金属微晶有限地递送到室温下。 AT结构揭示了该核心PKS酶的重要催化特征，包括在活性位点周围的构象变化发生。讨论了聚醚合酶反应动力学对这些构象变化的影响。

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《Biochemistry》 |2017年第36期|共6页
作者
Mathews Irimpan I.; Allison Kim; Robbins Thomas; Lyubimov Artem Y.; Uervirojnangkoorn Monarin; Brunger Axel T.; Khosla Chaitan; DeMirci Hasan; McPhillips Scott E.; Hollenbeck Michael; Soltis Michael; Cohen Aina E.;
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Stanford Synchrotron Radiat Lightsource 2575 Sand Hill Rd Menlo Pk CA 94025 USA;

Stanford Synchrotron Radiat Lightsource 2575 Sand Hill Rd Menlo Pk CA 94025 USA;

Stanford Univ Dept Chem Stanford CA 94305 USA;

Stanford Synchrotron Radiat Lightsource 2575 Sand Hill Rd Menlo Pk CA 94025 USA;

Stanford Univ Dept Mol &

Cellular Physiol Stanford CA 94305 USA;

Stanford Univ Dept Mol &

Cellular Physiol Stanford CA 94305 USA;

Stanford Univ Dept Chem Stanford CA 94305 USA;

Stanford Synchrotron Radiat Lightsource 2575 Sand Hill Rd Menlo Pk CA 94025 USA;

Stanford Synchrotron Radiat Lightsource 2575 Sand Hill Rd Menlo Pk CA 94025 USA;

Stanford Synchrotron Radiat Lightsource 2575 Sand Hill Rd Menlo Pk CA 94025 USA;

Stanford Synchrotron Radiat Lightsource 2575 Sand Hill Rd Menlo Pk CA 94025 USA;

Stanford Synchrotron Radiat Lightsource 2575 Sand Hill Rd Menlo Pk CA 94025 USA;

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中图分类生物化学;
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1. The Conformational Flexibility of the Acyltransferase from the Disorazole Polyketide Synthase Is Revealed by an X-ray Free-Electron Laser Using a Room-Temperature Sample Delivery Method for Serial Crystallography [J] . Mathews Irimpan I., Allison Kim, Robbins Thomas, Biochemistry . 2017,第36期

机译：通过使用用于连续晶体学的室温样品递送方法，通过X射线自由电子激光器揭示来自散唑聚酮合成酶的酰基转移酶的构象柔韧性
2. Multifarious injection chamber for molecular structure study (MICOSS) system: development and application for serial femtosecond crystallography at Pohang Accelerator Laboratory X-ray Free-Electron Laser [J] . Jaehyun Park, Seonghan Kim, Sangsoo Kim, Journal of synchrotron radiation . 2018,第2期

机译：用于分子结构研究的多种注射室（MICOSS）系统：浦项加速器实验室X射线自由电子激光器的串行飞秒晶体学的开发与应用
3. Trace phase detection and strain characterization from serial X-ray free-electron laser crystallography of a Pr0.5Ca0.5MnO3 powder [J] . Beyerlein Kenneth R., Jooss Christian, Barty Anton, Powder diffraction . 2015,第junasuppla1期

机译：Pr0.5Ca0.5MnO3粉末的连续X射线自由电子激光结晶学进行痕量相检测和应变表征
4. Redox dependent conformational change of the 2Fe-2Sferredoxin revealed by ultrahigh resolution X-ray crystallography [C] . YusukeOhnishi, Hideaki Tanaka, Hideo Okumura 日本化学会春季年会 . 2020

机译：通过超高分辨率X射线晶体表现出2FE-2S富勒森蛋白的氧化还原依赖性构象变化
5. Sample Injector Fabrication and Delivery Method Development for Serial Crystallography using Synchrotrons and X-ray Free Electron Lasers. [D] . Nelson, Garrett Charles. 2015

机译：使用同步加速器和无X射线电子激光进行串行晶体学的样品注射器制造和传输方法开发。
6. The Conformational Flexibility of the Acyltransferase from the Disorazole Polyketide Synthase Is Revealed by an X-ray Free-Electron Laser Using a Room-Temperature Sample Delivery Method for Serial Crystallography [O] . Irimpan I. Mathews, Kim Allison, Thomas Robbins, -1

机译：X-射线自由电子激光使用室温样品传递方法进行连续结晶学研究揭示了二甲苯唑聚酮化合物合酶中酰基转移酶的构象柔性。
7. Diverse application platform for hard X-ray diffraction in SACLA (DAPHNIS): application to serial protein crystallography using an X-ray free-electron laser. [O] . Tono, Kensuke, Nango, Eriko, Sugahara, Michihiro, 2015

机译：SACLA（DAPHNIS）中用于硬X射线衍射的多样化应用平台：使用X射线自由电子激光应用于串行蛋白质晶体学。

The Conformational Flexibility of the Acyltransferase from the Disorazole Polyketide Synthase Is Revealed by an X-ray Free-Electron Laser Using a Room-Temperature Sample Delivery Method for Serial Crystallography

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