Multiple Parameter Optimization for Maximization of Pectinase Production by Rhizopus sp. C4 under Solid State Fermentation

Shweta Handa; Nivedita Sharma; Shruti Pathania

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Multiple Parameter Optimization for Maximization of Pectinase Production by Rhizopus sp. C4 under Solid State Fermentation

机译：多参数优化可最大化根霉菌种产生果胶酶的能力。固态发酵下的C4

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A novel strain Rhizopus sp. C4 was isolated from compost for the production of pectinase. Cultivation of Rhizopus sp. C4 on orange peel substrate under various solid-state fermentation (SSF) conditions was evaluated for pectinase yield along with the enzyme activity profile as a potential, low-cost alternative to submerged-liquid fermentation. Response surface methodology (RSM) was employed to optimize various environmental parameters for pectinase production. Various parameters, namely temperature, moisture and incubation days, were studied statistically for a total of 20 runs using central composite design. The highest yield of the enzyme, i.e. , 11.63 IU/mL, was obtained from 1:3.5 moisture ratios in 7 days at 30°C. The study demonstrated that optimization through RSM could improve the enzymatic characteristics and yield of the enzyme.

机译：一种新型菌株根霉。从堆肥中分离出C4以产生果胶酶。根霉的栽培对果皮酶在各种固态发酵（SSF）条件下在果皮基质上的C4进行了果胶酶产量以及酶活性谱评估，以此作为淹没液体发酵的潜在低成本替代品。响应面方法（RSM）用于优化果胶酶生产的各种环境参数。使用中央复合设计对总共20次运行的各种参数（即温度，湿度和孵育天数）进行了统计研究。该酶的最高产量，即11.63 IU / mL，是在30℃下从7天的1：3.5水分比获得的。研究表明，通过RSM进行优化可以改善酶的酶学特性和产率。

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《Fermentation》 |2016年第2期|共页
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Shweta Handa; Nivedita Sharma; Shruti Pathania;
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1. Optimization of some parameters of solid state fermentation of wheat bran for protease production by a local strain of Rhizopus oryzae [J] . ALKAT K., BHATTACHARYYA B. C. Acta Biotechnologica . 2000,第2期

机译：米糠根霉菌局部发酵生产麸皮蛋白酶的一些参数的优化
2. Production and optimization of process parameters for alkaline protease production by a newly isolated Bacillus sp. under solid state fermentation [J] . Fikret Uyar, Zuebeyde Baysal Process Biochemistry . 2004,第12期

机译：通过新分离的芽孢杆菌属菌株生产和优化碱性蛋白酶生产工艺参数。固态发酵
3. Production and optimization of process parameters for alkaline protease production by a newly isolated Bacillus sp. under solid state fermentation [J] . Fikret Uyar, Zuebeyde Baysal Process Biochemistry . 2004,第12期

机译：通过新分离的芽孢杆菌属菌株生产和优化碱性蛋白酶生产工艺参数。固态发酵
4. Utilization of coffee pulp as a substrate for pectinase production by Aspergillus sp. VTMS through solid state fermentation [C] . Atim A. Hidayah, Azizah, Rudju Winarsa, International Conference on Science and Applied Science . 2020

机译：用茶虫SP用咖啡纸浆作为果胶酶产生的基材。 VTM通过固态发酵
5. Co-production of fumaric acid and chitin using Rhizopus oryzae fermentation on a nitrogen-rich agricultural residue: Dairy manure. [D] . Liao, Wei. 2005

机译：使用米根霉在富含氮的农业残留物上发酵生产富马酸和甲壳质：乳牛粪。
6. Optimization of thermostable organic solvent-tolerant lipase production by thermotolerant Rhizopus sp. using solid-state fermentation of palm kernel cake [O] . Fatimah Azizah Riyadi, Md. Zahangir Alam, Md. Noor Salleh, 2017

机译：耐热Rhizopus sp。生产耐热性有机溶剂耐受脂肪酶的优化。利用棕榈仁饼的固态发酵
7. Multiple Parameter Optimization for Maximization of Pectinase Production by Rhizopus sp. C4 under Solid State Fermentation [O] . Shweta Handa, Nivedita Sharma, Shruti Pathania 2016

机译：rhizopus sp果胶酶产生的多种参数优化。 C4在固态发酵下

Multiple Parameter Optimization for Maximization of Pectinase Production by Rhizopus sp. C4 under Solid State Fermentation

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