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Batch Fermentation Options for High Titer Bioethanol Production from a SPORL Pretreated Douglas-Fir Forest Residue without Detoxification

机译:从SPORL预处理的道格拉斯冷杉杉木残渣中进行高滴度生物乙醇生产的分批发酵选项,无需排毒

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This study evaluated batch fermentation modes, namely, separate hydrolysis and fermentation (SHF), quasi-simultaneous saccharification and fermentation (Q-SSF), and simultaneous saccharification and fermentation (SSF), and fermentation conditions, i.e., enzyme and yeast loadings, nutrient supplementation and sterilization, on high titer bioethanol production from SPORL-pretreated Douglas-fir forest residue without detoxification. The results indicated that Q-SSF and SSF were obviously superior to SHF operation in terms of ethanol yield. Enzyme loading had a strong positive correlation with ethanol yield in the range studied. Nutrient supplementation and sterility were not necessary for ethanol production from SPORL-pretreated Douglas-fir. Yeast loading had no substantial influence on ethanol yield for typical SSF conditions. After 96 h fermentation at 38 °C on shake flask at 150 rpm, terminal ethanol titer of 43.2 g/L, or 75.1% theoretical based on untreated feedstock glucan, mannan, and xylan content was achieved, when SSF was conducted at whole slurry solids loading of 15% with enzyme and yeast loading of 20 FPU/g glucan and 1.8 g/kg (wet), respectively, without nutrition supplementation and sterilization. It is believed that with mechanical mixing, enzyme loading can be reduced without reducing ethanol yield with extended fermentation duration.
机译:这项研究评估了分批发酵模式,即分别的水解和发酵(SHF),准同时糖化和发酵(Q-SSF),同时糖化和发酵(SSF)以及发酵条件,即酶和酵母的含量,营养成分补充和灭菌后,无需排毒即可从SPORL预处理的道格拉斯冷杉森林残留物中高滴度生产生物乙醇。结果表明,就乙醇收率而言,Q-SSF和SSF明显优于SHF操作。在所研究的范围内,酶的负载与乙醇的产量有很强的正相关。从SPORL预处理的道格拉斯冷杉生产乙醇不需要补充营养和无菌。在典型的SSF条件下,酵母菌的装载量对乙醇产量没有实质性影响。在38°C的摇瓶中以150 rpm发酵96小时后,当以全浆固体进行SSF时,最终乙醇滴定度为43.2 g / L,或理论值的75.1%,基于未处理的原料葡聚糖,甘露聚糖和木聚糖含量不添加营养和灭菌的情况下,酶含量分别为15%和20 FPU / g葡聚糖和1.8 g / kg(湿)的酵母。据信,通过机械混合,可以在不延长发酵持续时间的情况下降低酶负载而不降低乙醇产量。

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