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首页> 外文期刊>Fermentation >Simultaneous Bioconversion of Gelatinized Starchy Waste from the Rice Noodle Manufacturing Process to Lactic Acid and Maltose-Forming α-Amylase by Lactobacillus plantarum S21, Using a Low-Cost Medium
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Simultaneous Bioconversion of Gelatinized Starchy Waste from the Rice Noodle Manufacturing Process to Lactic Acid and Maltose-Forming α-Amylase by Lactobacillus plantarum S21, Using a Low-Cost Medium

机译：利用低成本培养基将米粉生产过程中的糊化淀粉废物同时生物转化为植物乳杆菌S21转化为乳酸和麦芽糖形成α-淀粉酶

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A direct bioconversion of gelatinized starchy waste (GSW) to lactic acid by amylolytic lactic acid bacterium Lactobacillus plantarum S21 was investigated. Corn steep liquor (CSL) was selected as the most suitable low-cost nitrogen source for replacing yeast extract, beef extract, and peptone in De Man, Rogosa and Sharpe (MRS) medium. Plackett–Burman design results indicated that GSW and CSL were the two most nutrients that significantly influence lactic acid production, among eight medium components, including GSW, CSL, K 2 HPO 4 , CH 3 COONa, (NH 4 ) 2 HC 6 H 5 O 7 , MgSO 4 , MnSO 4 , and Tween 80. A new low-cost medium containing only GSW (134.4 g/L) and CSL (187.7 g/L) was achieved as omitting other six components from the optimized medium had no effect on lactic acid yield. Batch fermentation at 37 °C both in 1 L and 10 L jar fermenters showed non-significantly different productivity. A by-product, maltose-forming α-amylase, was successfully achieved up to 96% recovery yield using an ultrafiltration unit equipped with a 50 kDa cut-off membrane. Crude lactic acid exhibited the additional benefit of antimicrobial activity against food and feed pathogens Salmonella enterica serovar Typhimurium TISTR 292, Vibrio cholerae TH-001, and also E. coli ATCC 25922. This study presents a promising bioprocess for the simultaneous production of lactic acid, and a value-added food enzyme, using only two industrial wastes, GSW and CSL, as the medium components.
机译：研究了淀粉分解的乳酸菌植物乳杆菌S21将糊化的淀粉废物（GSW）直接生物转化为乳酸。在德曼，罗戈萨和夏普（MRS）培养基中，玉米浆（CSL）被选为最适合替代酵母提取物，牛肉提取物和蛋白ept的低成本氮源。 Plackett-Burman设计结果表明，GSW和CSL是显着影响乳酸生产的两种最重要的营养素，在GSW，CSL，K 2 HPO 4，CH 3 COONa，（NH 4）2 HC 6 H 5等八种培养基成分中O 7，MgSO 4，MnSO 4和Tween80。获得了仅包含GSW（134.4 g / L）和CSL（187.7 g / L）的新型低成本培养基，因为从优化的培养基中省略了其他六种成分没有效果乳酸的产量。在1升和10升广口瓶发酵罐中，在37°C下分批发酵显示出无显着差异的生产率。使用配备了50 kDa截止膜的超滤装置，成功地实现了副产物麦芽糖形成α-淀粉酶的回收，回收率高达96％。粗乳酸显示出对食品和饲料病原体的抗菌活性的附加优势。肠炎沙门氏菌鼠伤寒沙门氏菌TISTR 292，霍乱弧菌TH-001以及大肠杆菌ATCC25922。这项研究为同时生产乳酸，仅使用两种工业废料（GSW和CSL）作为介质成分的增值食品酶。

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