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Assessment of the Effects of Chemotherapy on Ovarian Reserve in Rats

机译：化学疗法对大鼠卵巢储备的影响评估

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OBJECTIVE: To determine the effect of cyclophosphamide and cisplatin on ovarian reserve in rats by?the way of counting primordial follicle and assessing TNF-alpha, 8-OH deguanosine and oxidant/antioxidant status. STUDY DESIGN: Thirty Wistar rats were divided into three groups. Cisplatin was administered at a dose?of 5 mg/kg (Cisplatin group, n=10), cyclophosphamide was administered at a dose of 6 mg/kg?(Cyclophosphamide group, n=10) and control group was only given sterile saline (Control group, n=10)?in Ege University Faculty of Medicine. All injections were administered intraperitoneally at a single dose.?At the end of the 7th day blood samples were drawn by intracardiac aspiration and bilateral extirpation?of ovaries were performed. The number of primordial follicles, levels of TNF-alpha, 8-OH deoxyguanosine,?malondialdehyde (MDA) and catalase enzyme activities were evaluated. RESULTS: The number of primordial follicles in the control group, cisplatin group and cyclophosphamide?group were 27.5±4.7;17.3±2.1;17±1.4, respectively (p=0.156). While the levels of TNF-alpha?were significantly higher in cisplatin group (p0,001), 8-OH deoxyguanosine levels did not have significant?difference among three groups (p=0.431). The levels of MDA were significantly lower compared to the other groups (p0.001); however, the catalase enzyme activities did not show statistically?significant difference. CONCLUSIONS: Insignificant depletion of primordial follicles was observed by using specified doses of?chemotherapeutics. An increase in TNF-alpha and MDA levels may play a role in the development of?ovarian tissue damage.
机译：目的：通过计数原始卵泡，评估TNF-α，8-OH脱鸟苷和氧化剂/抗氧化剂的状态，确定环磷酰胺和顺铂对大鼠卵巢储备的影响。研究设计：将三十只Wistar大鼠分为三组。顺铂的剂量为5 mg / kg？（顺铂组，n = 10），环磷酰胺的剂量为6 mg / kg？（环磷酰胺组，n = 10），对照组仅给予无菌生理盐水（对照组，n = 10）？在Ege大学医学院。所有注射均以单剂量腹膜内给药。在第7天结束时，通过心内穿刺抽血并进行卵巢双侧切除术。评价了原始卵泡的数目，TNF-α，8-OH脱氧鸟苷，β-丙二醛（MDA）和过氧化氢酶的活性。结果：对照组，顺铂组和环磷酰胺？组的原始卵泡数分别为27.5±4.7; 17.3±2.1; 17±1.4（p = 0.156）。顺铂组的TNF-α水平显着升高（p <0.001），而三组之间的8-OH脱氧鸟苷水平没有显着性差异（p = 0.431）。与其他组相比，MDA的水平显着降低（p <0.001）。然而，过氧化氢酶的活性没有统计学上的显着差异。结论：通过使用指定剂量的化学治疗剂，观察到原始卵泡的耗竭很小。 TNF-α和MDA水平的升高可能在卵巢组织损伤的发展中起作用。

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