Assessment of the levels of nitric oxide (NO) and cytokines (IL-5, IL-6, IL-13, TNF, IFN-gamma) in giardiosis

Joanna Matowicka-Karna; Maciej Kralisz; Halina Kemona

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Assessment of the levels of nitric oxide (NO) and cytokines (IL-5, IL-6, IL-13, TNF, IFN-gamma) in giardiosis

机译：评估贾第氏病中一氧化氮（NO）和细胞因子（IL-5，IL-6，IL-13，TNF，IFN-γ）的水平

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The current study aims to determine the involvement of cellular responses in combating Giardia intestinalis invasion. The study group consisted of 44 women and 18 men, aged 18–72 years, infected with G. intestinalis. The diagnosis was established based on laboratory investigations (examination of stool, choloscopy, GSA-65). Blood for analysis was collected before antiparasitic treatment and two weeks after treatment termination. The control group consisted of 22 women and 18 men aged 20–45 years. The serum concentrations of IL-5, IL-6, IL-13, TNF, IFN-γ were assayed using a set of Quantikine human. The concentrations of NO in the serum were determined using a set of Total Nitric Oxide Assay. Patients infected with G. intestinalis showed a statistically significant increase in the levels of NO, IFN-g and IL-13. Even the antiparasitic treatment applied did not reduce the levels of these parameters and only caused a rise in IL-6. Our study showed a lack of acute inflammatory state in the course of G. intestinalis infection. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 2, pp. 280–284)

机译：目前的研究旨在确定细胞反应在抵抗贾第鞭毛虫肠道入侵中的作用。该研究小组由44名女性和18名男性组成，年龄分别为18-72岁，并感染了肠道小肠结肠菌。诊断是根据实验室检查（粪便检查，胆管镜检查，GSA-65）确定的。抗寄生虫治疗前和治疗终止后两周收集血液进行分析。对照组包括22名女性和18名年龄在20-45岁之间的男性。使用一套Quantikine人测定了IL-5，IL-6，IL-13，TNF，IFN-γ的血清浓度。使用一套总一氧化氮测定法测定血清中NO的浓度。肠道小肠结肠菌感染的患者显示NO，IFN-g和IL-13的水平有统计学上的显着增加。即使采用抗寄生虫治疗也不会降低这些参数的水平，只会引起IL-6升高。我们的研究表明，肠球菌感染过程中缺乏急性炎症状态。（Folia Histochemica et Cytobiologica 2011;第49卷，第2期，第280-284页）

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《Folia histochemica et cytobiologica》 |2011年第2期|共5页
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Joanna Matowicka-Karna; Maciej Kralisz; Halina Kemona;
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1. Assessment of the levels of nitric oxide (NO) and cytokines (IL-5, IL-6, IL-13, TNF, IFN-gamma) in giardiosis [J] . Matowicka-KarnaJ., KraliszM., KemonaH. Folia histochemica et cytobiologica . 2011,第2期

机译：评估贾第氏病中一氧化氮（NO）和细胞因子（IL-5，IL-6，IL-13，TNF，IFN-γ）的水平
2. Assessment of the levels of nitric oxide (NO) and cytokines (IL-5, IL-6, IL-13, TNF, IFN-gamma) in giardiosis [J] . Joanna Matowicka-Karna, Maciej Kralisz, Halina Kemona Folia histochemica et cytobiologica . 2011,第2期

机译：评估贾第氏病中一氧化氮（NO）和细胞因子（IL-5，IL-6，IL-13，TNF，IFN-γ）的水平
3. Lactic acid bacteria enhance autophagic ability of mononuclear phagocytes by increasing Th1 autophagy-promoting cytokine (IFN-gamma) and nitric oxide (NO) levels and reducing Th2 autophagy-restraining cytokines (IL-4 and IL-13) in response to Mycobacterium tuberculosis antigen. [J] . Ghadimi D, de Vrese M, Heller KJ, International immunopharmacology . 2010,第6期

机译：乳酸菌通过响应结核分枝杆菌抗原增加Th1自噬促进细胞因子（IFN-γ）和一氧化氮（NO）的水平并降低Th2自噬抑制细胞因子（IL-4和IL-13）来增强单核吞噬细胞的自噬能力。。
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机译：氮化钛，硅化钛和碲化钛颗粒以及LPS在RAW 264.7小鼠巨噬细胞中诱导TNF-α，IL-6和一氧化氮
5. Mechanical-tactile stimulation in a neonatal stress model alters depot-specific expression of proinflammatory cytokines, TNF-α and IL-6 [D] . Warner, Grace 2011

机译：新生儿应激模型中的机械触觉刺激改变了促炎性细胞因子，TNF-α和IL-6的场特异性表达
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机译：弓形虫感染会影响IgE和细胞因子（IL-5IL-6IL-10IL-12和TNF-α）的水平吗？
7. Assessment of the levels of nitric oxide (NO) and cytokines (IL-5, IL-6, IL-13, TNF, IFN-gamma) in giardiosis [O] . Halina Kemona, Joanna Matowicka-Karna, Maciej Kralisz 2011

机译：评估心脏病中一氧化氮（NO）和细胞因子（IL-5，IL-6，IL-13，TNF，IFN-γ）的水平

Assessment of the levels of nitric oxide (NO) and cytokines (IL-5, IL-6, IL-13, TNF, IFN-gamma) in giardiosis

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