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首页> 外文期刊>Food Science & Nutrition >Extraction, purification, and structure characterization of polysaccharides from Crassostrea rivularis
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Extraction, purification, and structure characterization of polysaccharides from Crassostrea rivularis

机译：小叶景天多糖的提取，纯化及结构表征

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Crude polysaccharide was prepared from Crassostrea rivularis by 30% (w/v) potassium hydroxide solution at 90°C for 120?min. Three fractions (OG1, OG2, and OG3) were purified by DEAE‐52 cellulose and Sepharose 2B gel column chromatography. The chemical structures were determined using gas chromatography (GC), high‐performance gel permeation chromatography (HPGPC), Fourier‐transform infrared (FT‐IR) spectroscopy, and 1 H and 13 C nuclear magnetic resonance (NMR) spectroscopy. The results indicated that OG1 was composed of rhamnose and little mannose (8.71%), the ratio of Rha: Gal: Xyl: Fuc in OG3 were 14:5.5:3:1. And their average molecular weights (Mw) were about 1.66?×?10 6 and 2.33?×?10 6 ?Da, respectively. OG2 was composed only of glucose (98.23%), which means it was glycogen. OG2 was consisted mainly of →4)‐ α‐D‐Glc‐(1→, with the branch chain every 6.5 glucose residues on average, which is →4,6)‐α‐D‐Glc‐(1→ and trace amount of α‐D‐Glc‐(1→ branched units. The Mw was 2.27?×?10 6 ?Da. It provides the bases for the bioactivity research.
机译：在90°C下用30％（w / v）氢氧化钾溶液从小叶景天藻中制备粗多糖120？min。通过DEAE-52纤维素和Sepharose 2B凝胶柱色谱纯化了三部分（OG1，OG2和OG3）。使用气相色谱（GC），高性能凝胶渗透色谱（HPGPC），傅立叶变换红外（FT-IR）光谱以及1 H和13 C核磁共振（NMR）光谱确定化学结构。结果表明，OG1由鼠李糖和少量甘露糖组成（8.71％），OG3中Rha：Gal：Xyl：Fuc的比例为14：5.5：3：1。并且它们的平均分子量（Mw）分别为约1.66Ω×≤106和2.33Ω×≤106ΩDa。 OG2仅由葡萄糖组成（98.23％），这意味着它是糖原。 OG2主要由→4）‐ α‐D‐Glc‐（1→组成，平均每6.5个葡萄糖残基分支链，即→4,6）‐α‐D‐Glc‐（1→和痕量α-D-Glc-（1→分支单元）的分子量为2.27？×？10 6？Da，为生物活性研究提供了依据。

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