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首页> 外文期刊>Folia histochemica et cytobiologica >Inverse relationship between TCTP/RhoA and p53/ /cyclin A/actin expression in ovarian cancer cells
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Inverse relationship between TCTP/RhoA and p53/ /cyclin A/actin expression in ovarian cancer cells

机译：卵巢癌细胞中TCTP / RhoA与p53 // cyclin A /肌动蛋白表达的负相关

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The translationally controlled tumor protein (TCTP) plays a role in cell growth, cell cycle and cancer progression. TCTP controls negatively the stability of the p53 tumor suppressor protein and interacts with the cellular cytoskeleton. The deregulation of the actin and cytokeratin cytoskeleton is responsible for the increased migratory activity of tumor cells and is linked with poor patient outcome. Recent studies indicate that cyclin A, a key regulator of cell cycle, controls actin organization and negatively regulates cell motility via regulation of RhoA expression. We studied the organization of actin and cytokeratin cytoskeleton and the expression of TCTP, p53, cyclin A, RhoA and actin in HIO180 non-transformed ovarian epithelial cells, and OVCAR3 and SKOV3 (expressing low level of inducible p53) ovarian epithelial cancer cells with different metastatic potential. Immunostaining and ultrastructural analyses illustrated a dramatic difference in the organization of the cytokeratin and actin filaments in non-transformed versus cancer cell lines. We also determined that there is an inverse relationship between the level of TCTP/RhoA and actin/p53/cyclin A expression in ovarian cancer cell lines. This previously unidentified negative relationship between TCTP/RhoA and actin/p53/cyclin A may suggest that this interaction is linked with the high aggressiveness of ovarian cancers.
机译：翻译控制的肿瘤蛋白（TCTP）在细胞生长，细胞周期和癌症进展中起作用。 TCTP负面地控制p53肿瘤抑制蛋白的稳定性，并与细胞的细胞骨架相互作用。肌动蛋白和细胞角蛋白细胞骨架的失调是导致肿瘤细胞迁移活动增加的原因，并且与患者预后不良有关。最近的研究表明，细胞周期蛋白的关键调节剂细胞周期蛋白A控制肌动蛋白的组织，并通过调节RhoA表达来负调节细胞运动能力。我们研究了肌动蛋白和细胞角蛋白细胞骨架的组织以及TCTP，p53，cyclin A，RhoA和肌动蛋白在HIO180未转化的卵巢上皮细胞以及OVCAR3和SKOV3（表达低水平的可诱导性p53）卵巢上皮癌细胞中的表达。转移潜力。免疫染色和超微结构分析显示了非转化细胞系和癌细胞系中细胞角蛋白和肌动蛋白丝组织的显着差异。我们还确定，在卵巢癌细胞系中TCTP / RhoA水平与肌动蛋白/ p53 /细胞周期蛋白A表达之间存在反比关系。 TCTP / RhoA和肌动蛋白/ p53 /细胞周期蛋白A之间这种先前未知的负相关关系可能表明这种相互作用与卵巢癌的高侵袭性有关。

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