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首页> 外文期刊>Food Science & Nutrition >Effects of age and extraction solvent on phytochemical content and antioxidant activity of fresh Moringa oleifera L. leaves
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Effects of age and extraction solvent on phytochemical content and antioxidant activity of fresh Moringa oleifera L. leaves

机译：年龄和提取溶剂对新鲜辣木叶片理化含量和抗氧化活性的影响

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Antioxidant activity (AOA) and phytochemical content of Moringa oleifera Lam leaves were determined as a function of their age and extraction solvent. Fresh Moringa leaves aged 30, 45, and 60?days were harvested and extracted with three solvents; methanol, ethanol, and water. AOA of leaf extracts was measured using radical scavenging assays (DPPH, ABTS, antiperoxide activity [APA]) and reducing assays (FRAP and total antioxidant capacity [TAC]), and these were correlated with total polyphenols (TPC), total flavonoids (TFC), and chlorophyll contents of leaves. Significant variability ( p ??0.05) in TPC and AOA of Moringa leaf extracts was observed with age and extraction solvent as well as their interaction. TPC and TFC increased with maturity, except in aqueous extract. The 60‐day‐old leaves showed highest TPC, TFC, and tocopherol contents with highest DPPH activity. On their part, 30‐day‐old leaves recorded better vitamin C, chlorophyll, and carotenoids with highest ABTS activity and APA. Methanol was best extraction solvent for TPC (4.6?g GAE/100?g DM) while ethanol was for flavonoids (1.8?g CE/100?g DM). Ethanol extracts exhibited the highest DPPH activity (53.3%–71.1%), while both ethanolic and methanolic extracts had similar and higher ABTS + activity (3.83–3.86?g AAE/100?g DM). Strong positive correlations ( r ?≥?0.8; p ??0.05) were observed between chlorophyll content and DPPH, ABTS, and APA, suggesting that chlorophyll was the major contributor to AOA. TAC was highest in aqueous solvent. Free radical scavenging activity in Moringa leaves is positively correlated to chlorophyll, TFC, and TPC while reducing power is positively correlated to chlorophyll and TPC. AOA of fresh Moringa leaf extract is a function of its phytochemical content and is influenced by both the age of the leaves and the extraction solvent used. Methanolic and ethanolic extracts of 45‐day‐old Moringa leaves exhibited best antioxidant potentials.
机译：测定辣木叶片的抗氧化活性（AOA）和植物化学含量，取决于其年龄和提取溶剂。收获30、45和60天的新鲜辣木叶，并用三种溶剂萃取。甲醇，乙醇和水。使用自由基清除试验（DPPH，ABTS，抗过氧化物活性[APA]）和还原试验（FRAP和总抗氧化剂能力[TAC]）测量叶片提取物的AOA，并将其与总多酚（TPC），总黄酮（TFC）相关）和叶子的叶绿素含量。观察到辣木叶片提取物的TPC和AOA随年龄和提取溶剂及其相互作用的显着变化（p≤0.05）。 TPC和TFC随成熟度增加而增加，除了在水提取物中。 60天大的叶片显示出最高的TPC，TFC和生育酚含量，DPPH活性最高。就其而言，30天的叶子记录了更好的维生素C，叶绿素和类胡萝卜素，具有最高的ABTS活性和APA。甲醇是TPC的最佳萃取溶剂（4.6？g GAE / 100？g DM），而乙醇是黄酮类化合物（1.8？g CE / 100？g DM）。乙醇提取物表现出最高的DPPH活性（53.3％–71.1％），而乙醇和甲醇提取物具有相似且更高的ABTS +活性（3.83–3.86？g AAE / 100？g DM）。叶绿素含量与DPPH，ABTS和APA之间观察到强正相关（r≥≥0.8;p≤0.05），表明叶绿素是AOA的主要贡献者。 TAC在水性溶剂中最高。辣木叶中的自由基清除活性与叶绿素，TFC和TPC正相关，而降低功率与叶绿素和TPC正相关。新鲜辣木叶提取物的AOA是其植物化学含量的函数，并且受叶龄和所用提取溶剂的影响。 45天龄的辣木叶的甲醇和乙醇提取物表现出最佳的抗氧化潜力。

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