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首页> 外文期刊>Folia histochemica et cytobiologica >Selective gene expression profiling of mTOR-associated tumor suppressor and oncogenes in ovarian cancer
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Selective gene expression profiling of mTOR-associated tumor suppressor and oncogenes in ovarian cancer

机译：卵巢癌中mTOR相关肿瘤抑制基因和癌基因的选择性基因表达谱

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The aim of this study was to selectively profile the activation status of mammalian target of rapamycin (mTOR)-associated oncogenes and tumor suppressor genes (TSGs) in ovarian cancer specimens, healthy ovaries and benign ovarian tumors, including endometrial cysts. We used a novel type of microfluidic gene array to examine the expression of 15 human tumor suppressors and oncogenes in ovarian cancer specimens of 53 patients, benign ovarian cysts of 29 women (endometrial and simple) and 11 healthy ovaries of individuals in whom the material was obtained during total hysterectomies performed because of fibroid changes. The array was custom-designed to include the following genes: NF1, RHEB, mTOR1, AKT-1, PTEN, TSC1, TSC2, KRAS, RPS6KB1, 4EBP1, TP53, EIF4E, STK11, PIK3CA and BECN1. Confirmatory immunohistochemical detection was performed for a group of selected proteins. Particularly significant differences were observed as to the expression of PTEN (p < 0.0001), TP53 (p = 0.0003), PIK3CA (p = 0.0003) and BECN1 (p = 0.0014) which were shown to be downregulated in cancer patients when compared to healthy ovaries and benign ovarian cysts (endometrial and simple). These markers did not show association with grade or stage of the tumor. Immunohistochemistry showed that PTEN, TP53, PIK3CA and BECN1 proteins are expressed in ovarian cancer. Our results indicate that there are significant differences in the expression of some of the mTOR-related tumor suppressors and oncogenes which could be associated with the pathogenesis of ovarian cancer. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 2, pp. 317–324)
机译：这项研究的目的是在卵巢癌标本，健康卵巢和良性卵巢肿瘤（包括子宫内膜囊肿）中选择性分析哺乳动物雷帕霉素（mTOR）相关靶基因和肿瘤抑制基因（TSG）的激活状态。我们使用一种新型的微流控基因阵列检查了15例人类肿瘤抑制因子和癌基因在53例卵巢癌标本，29例女性（子宫内膜和单纯性）良性卵巢囊肿以及11例健康卵巢中的表达。因子宫肌瘤改变而在进行全子宫切除术时获得的结果。该阵列经过定制设计，包括以下基因：NF1，RHEB，mTOR1，AKT-1，PTEN，TSC1，TSC2，KRAS，RPS6KB1、4EBP1，TP53，EIF4E，STK11，PIK3CA和BECN1。对一组选定的蛋白质进行了确认性免疫组织化学检测。与癌症患者相比，PTEN（p <0.0001），TP53（p = 0.0003），PIK3CA（p = 0.0003）和BECN1（p = 0.0014）的表达特别显着差异。卵巢和卵巢良性囊肿（子宫内膜和单纯性）。这些标志物未显示与肿瘤的等级或阶段相关。免疫组织化学表明，PTEN，TP53，PIK3CA和BECN1蛋白在卵巢癌中表达。我们的结果表明，某些与mTOR相关的肿瘤抑制因子和癌基因的表达存在显着差异，这可能与卵巢癌的发病机理有关。 （Folia Histochemica et Cytobiologica 2011;第49卷，第2期，第317–324页）

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