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首页> 外文期刊>Folia histochemica et cytobiologica >Involucrin, but not filaggrin and Kdap mRNA, expression is downregulated in 3-D cultures of intact rat hair bulbs after calcium stimulation
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Involucrin, but not filaggrin and Kdap mRNA, expression is downregulated in 3-D cultures of intact rat hair bulbs after calcium stimulation

机译：钙刺激后，完整大鼠毛鳞球的3-D培养物中的蛋清蛋白而不是丝蛋白和Kdap mRNA的表达下调

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The hair follicle consists of several distinctive epidermal cell layers. The hair root, which undergoes keratinization, is surrounded by two sheaths: the inner root sheath (IRS) and the outer root sheath (ORS). The ORS is continuous with the basal layer of the epidermis. Its cells do not keratinize in situ , unlike IRS. We have previously demonstrated that keratinization of the ORS was prevented by contact with the IRS in hair follicle mid-segments (i.e. fragments dissected from skin at the level above the hair bulb and below the opening of the sebaceous gland duct) cultured on agarose layer. The purpose of this study was to determine whether the same applies to the hair bulb. After isolation, intact bulbs or hair bulb-derived cells were incubated in suspension in a low or high calcium medium. The level of mRNA for differentiation markers: involucrin, filaggrin, keratinocyte differentiation associated protein and trichohyalin, was studied by RealTime PCR. We observed increased Ca 2+ upregulated expression of involucrin, filaggrin, trichohyalin and Kdap in cultures of bulb-derived cells, but in hair bulbs downregulation of involucrin and trichohyalin was observed. We concluded that the inner root sheath exerts an inhibitory effect on the expression of involucrin and trichohyalin already in the hair bulbs. The observation that downregulation of involucrin expression under Ca 2+ influence occurs both in hair bulb and midsegments could simplify future experiments, since their separation does not seem to be necessary. (Folia Histochemica et Cytobiologica 2011, Vol. 49, No. 2, 335–343)
机译：毛囊由几个独特的表皮细胞层组成。经历角化的发根被两个鞘包围：内根鞘（IRS）和外根鞘（ORS）。 ORS与表皮基底层连续。与IRS不同，它的细胞不会原位角化。我们以前已经证明，通过在琼脂糖层上培养的毛囊中段（即从毛囊以上和皮脂腺管的开口以下的水平从皮肤上切下的片段）与IRS接触可以防止ORS的角化。这项研究的目的是确定是否同样适用于毛囊。分离后，将完整的鳞茎或鳞茎来源的细胞在低钙或高钙培养基中悬浮培养。通过实时荧光定量PCR（RealTime PCR）研究了分化标记物：整合素，丝蛋白，角质形成细胞分化相关蛋白和毛滴虫的mRNA水平。我们观察到在鳞茎来源的细胞培养物中，Inluclucrin，丝聚蛋白，trichohyalin和Kdap的Ca 2+上调表达增加，但在毛鳞茎中观察到Inluclucrin和Trichohyalin的下调。我们得出的结论是，内根鞘对已经存在于毛发鳞茎中的总蛋白和三氯透明质蛋白表达具有抑制作用。在Ca 2+的影响下，整合素表达的下调在毛鳞茎和中段都发生的观察结果可以简化未来的实验，因为它们的分离似乎不是必需的。 （Folia Histochemica et Cytobiologica 2011，Vol.49，No.2，335-343）

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