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首页> 外文期刊>Folia histochemica et cytobiologica >Distribution and chemical coding of neurons in intramural ganglia of the porcine urinary bladder trigone.
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Distribution and chemical coding of neurons in intramural ganglia of the porcine urinary bladder trigone.

机译:猪膀胱三角骨壁内神经节中神经元的分布和化学编码。

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This study presents the distribution and chemical coding of neurons in the porcine intramural ganglia of the urinary bladder trigone (IG-UBT) demonstrated using combined retrograde tracing and double-labelling immunohistochemistry. Retrograde fluorescent tracer Fast Blue (FB) was injected into the wall of both the left and right side of the bladder trigone during laparotomy performed under pentobarbital anaesthesia. Ten-microm-thick cryostat sections were processed for double-labelling immunofluorescence with antibodies against tyrosine hydroxylase (TH), dopamine beta-hydroxylase (DBH), neuropeptide Y (NPY), somatostatin (SOM), galanin (GAL), vasoactive intestinal polypeptide (VIP), nitric oxide synthase (NOS), calcitonin gene-related peptide (CGRP), substance P (SP), Leu5-enkephalin (LENK) and choline acetyltransferase (ChAT). IG-UBT neurons formed characteristic clusters (from a few to tens neuronal cells) found under visceral peritoneum or in the outer muscular layer. Immunohistochemistry revealed four main populations of IG-UBT neurons: SOM- (ca. 35%), SP- (ca. 32%), ChAT- and NPY- immunoreactive (-IR) (ca. 23%) as well as non-adrenergic non-cholinergic nerve cells (ca. 6%). This study has demonstrated a relatively large population of differently coded IG-UBT neurons, which constitute an important element of the complex neuro-endocrine system involved in the regulation of the porcine urogenital organ function.
机译:这项研究提出了使用逆行示踪和双重标记免疫组化技术证明的猪三角骨(IG-UBT)猪壁内神经节中神经元的分布和化学编码。在戊巴比妥麻醉下进行剖腹手术的过程中,逆行荧光示踪剂Fast Blue(FB)被注入膀胱三角区的左侧和右侧壁。使用酪氨酸羟化酶(TH),多巴胺β-羟化酶(DBH),神经肽Y(NPY),生长抑素(SOM),甘丙肽(GAL),血管活性肠多肽的抗体对10微米厚的低温恒温器切片进行双标记免疫荧光处理(VIP),一氧化氮合酶(NOS),降钙素基因相关肽(CGRP),P物质(SP),Leu5-脑啡肽(LENK)和胆碱乙酰基转移酶(ChAT)。 IG-UBT神经元形成了内脏腹膜下或外肌层中发现的特征簇(从几到数十个神经元细胞)。免疫组织化学显示IG-UBT神经元有四个主要种群:SOM-(约占35%),SP-(约占32%),ChAT-和NPY免疫反应性(-IR)(约23%)以及非肾上腺能非胆碱能神经细胞(约6%)。这项研究证明了相对大量的不同编码的IG-UBT神经元,它们构成了复杂的神经内分泌系统的重要元素,参与了泌尿生殖器官功能的调节。

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