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Effect of psyllium and gum Arabic biopolymers on the survival rate and storage stability in yogurt of Enterococcus durans IW3 encapsulated in alginate

机译:车前子和阿拉伯树胶生物聚合物对藻酸盐包埋的杜兰肠球菌IW3酸奶存活率和储存稳定性的影响

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Abstract Different herbal biopolymers were used to encapsulate Enterococcus durans IW3 to enhance its storage stability in yogurt and subsequently its endurance in gastrointestinal condition. Nine formulations of encapsulation were performed using alginate (ALG), ALG-psyllium (PSY), and ALG-gum Arabic (GA) blends. The encapsulation efficiency of all formulations, tolerance of encapsulated E. durans IW3 against low pH/high bile salt concentration, storage lifetime, and release profile of cells in natural condition of yogurt were evaluated. Result revealed 98.6% encapsulation efficiency and 76% survival rate for all formulation compared with the unencapsulated formulation cells (43%). The ALG-PSY and ALG-GA formulations have slightly higher survival rates at low pH and bile salt condition (i.e., 76?¢????93% and 81?¢????95%, respectively) compared with the ALG formulation. All encapsulated E. durans IW3 was released from the prepared beads of ALG after 90 min, whereas both probiotics encapsulated in ALG-GA and ALG-PSY were released after 60 min. Enterococcus durans IW3 was successfully encapsulated in ALG, ALG-GA, and ALG-PSY beads prepared by extrusion method. ALG-GA and ALG-PSY beads are suitable delivery carriers for the oral administration of bioactive compounds like probiotics. The GA and PSY gels exhibited better potential for encapsulation of probiotic bacteria cells because of the amendment of ALG difficulties and utilization of therapeutic and prebiotic potentials of these herbal biopolymers.
机译:摘要用不同的草药生物聚合物包囊杜伦肠球菌IW3,以增强其在酸奶中的储存稳定性,进而增强其在胃肠道条件下的耐力。使用藻酸盐(ALG),ALG-车前草(PSY)和ALG-阿拉伯胶(GA)混合物进行了9种封装配方。评价了所有制剂的包封效率,包封的杜鹃花IW3对低pH /高胆汁盐浓度的耐受性,储存寿命以及酸奶自然条件下细胞的释放曲线。结果显示,与未封装的配方细胞(43%)相比,所有配方的封装效率为98.6%,存活率为76%。与ALG相比,ALG-PSY和ALG-GA制剂在低pH和胆盐条件下的存活率略高(即分别为76%〜93%和81%〜95%)。公式。 90分钟后,所有制备好的ALG小珠均释放出所有包埋的杜鹃花IW3,而60分钟后释放出ALG-GA和ALG-PSY中的两种益生菌。杜兰肠球菌IW3已成功封装在通过挤压法制备的ALG,ALG-GA和ALG-PSY珠中。 ALG-GA和ALG-PSY磁珠是适合口服施用生物活性化合物(如益生菌)的载体。由于ALG困难的解决以及这些草药生物聚合物的治疗和益生元潜力的利用,GA和PSY凝胶在益生菌细胞的封装方面显示出更好的潜力。

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