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New insights on effects of a dietary supplement on oxidative and nitrosative stress in humans

机译：关于膳食补充剂对人体氧化和亚硝化应激影响的新见解

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AbstractThe research community is generally agreed that maintenance of healthy levels of free radicals and related oxidants are important for good health. However, utilization of the “redox stress hypothesis” can provide us with concrete nutritional targets in order to better support and maintain “optimal health.” Following this hypothesis we performed a crossover, double-blind, placebo-controlled, single-dose study on the effects of SPECTRA™, a dietary supplement, on oxidative stress markers (OSM) in human participants (n = 22). The measurement of OSM (ex vivo intra- and extracellular formation of reactive oxygen species (ROS, O2−, H2O2, OH−) in whole blood, respiratory activity of blood cells, as well as mitochondrial-dependent ROS formation, and respiratory activity), was performed using EPR spectrometer nOxyscan, spin probe CMH, and oxygen label NOX-15.1, respectively. Furthermore, we investigated the ability of SPECTRA™ to modulate ex vivo cellular inflammatory responses induced by stimulation with exogenous TNF-α and also followed changes in bioavailable NO concentrations. In this clinical study, we demonstrated that administration of SPECTRA™ resulted in statistically significant long-term inhibition of mitochondrial and cellular ROS generation by as much as 17% as well as 3.5-times inhibition in extracellular NADPH system-dependent generation of O2−, and nearly complete inhibition of extracellular H2O2 formation. This was reflected in more than two times inhibition of ex vivo cellular inflammatory response and also increases in bioavailable NO concentration. For the first time, we have measured synergetic, biological effects of a natural supplement on changes in OSM and cellular metabolic activity. The unique design and activity of the plant-based natural supplement, in combination with the newly developed and extended Vitality test, demonstrates the potential of using dietary supplements to modulate OSM and also opens the door to future research into the use of natural supplements for supporting optimal health.
机译：摘要研究界普遍认为，维持自由基和相关氧化剂的健康水平对健康至关重要。但是，利用“氧化还原应激假说”可以为我们提供具体的营养指标，以便更好地支持和维持“最佳健康”。根据这一假设，我们对饮食补充剂SPECTRA ™对人类参与者氧化应激指标（OSM）的影响进行了一项交叉，双盲，安慰剂对照，单剂量研究（n = 22）。 OSM的测量（活性氧（ROS，O 2 -，H 2 O 2 ，OH -），全血，呼吸细胞活性以及线粒体依赖性ROS的形成和呼吸活性）是使用EPR光谱仪nOxyscan，旋转探针CMH进行的和氧标签分别为NOX-15.1。此外，我们研究了SPECTRA ™调节外源性TNF-α刺激诱导的离体细胞炎症反应的能力，并且还跟踪了生物利用型NO浓度的变化。在这项临床研究中，我们证明了SPECTRA ™的给药在细胞外NADPH系统中对线粒体和细胞ROS产生的长期统计学抑制作用高达17％，是3.5倍抑制依赖的O 2 -生成，并几乎完全抑制细胞外H 2 O 2 的形成。这反映出对体内细胞炎症反应的抑制超过两倍，并且还增加了生物利用型NO的浓度。我们首次测量了天然补品对OSM和细胞代谢活性变化的协同生物学效应。基于植物的天然补充剂的独特设计和活性，与新开发和扩展的活力测试相结合，证明了使用膳食补充剂调节OSM的潜力，也为将来使用天然补充剂进行支持的研究打开了大门最佳健康。

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