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The Canadian Ophiostoma genome project

机译：加拿大蛇口骨基因组计划

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The Canadian Ophiostoma Genome Project, which was initiated in 2001, is a collaborative effort between research teams in four different universities. Its general objective is to conduct a large-scale identification and analysis of genes controlling important aspects of the life cycle of Ophiostomatoid fungi. To this end, several expressed sequence tag (EST) libraries were obtained for the Dutch elm disease pathogen Ophiostoma novo-ulmi and the sapstainer O. piceae, following partial, single-pass automated sequencing of complementary DNA clones. The largest EST library, prepared from yeast like cells of O. novo-ulmi grown at 24 °C, contains over 3,400 readable sequences and serves as a general reference library for Ophiostomatoid fungi. Smaller, specific EST libraries were constructed from mycelia of O. novo-ulmi grown at suboptimal temperatures, from perithecia formed in laboratory crosses, as well as from O. piceae grown on different carbon sources. Ongoing bioinformatic searches in public databases have so far identified over 750 Ophiostoma unique ESTs which show significant homology with other fungal genes of known function, although a high proportion of Ophiostoma ESTs are either orphans (no match to any known gene) or show homology to genes of unknown function. In addition to EST analysis, differential expression of selected genes and structural genomics are also being studied.
机译：加拿大卵母细胞瘤基因组计划于2001年启动，是四所不同大学的研究团队之间的共同努力。其总体目标是对控制蛇口骨类真菌生命周期重要方面的基因进行大规模鉴定和分析。为此，在对互补性DNA克隆进行部分，单次自动测序后，获得了荷兰榆病病原菌Ophiostoma novo-ulmi和sapstainer O. piceae的几个表达序列标签（EST）文库。最大的EST文库是由在24°C下生长的新乌尔莫斯酵母样细胞制备的，包含3,400多个可读序列，可作为蛇口骨类真菌的通用参考库。较小的，特定的EST文库是由在最适温度下生长的新乌尔姆菌丝体，实验室杂交形成的皮膜以及在不同碳源上生长的木虱组成的。迄今为止，在公共数据库中正在进行的生物信息学搜索已经鉴定出超过750种与已知功能的真菌基因显示出显着同源性的独特的EST，尽管大部分Ophiostoma EST是孤儿（与任何已知基因都不匹配）或与基因同源功能未知。除EST分析外，还研究了选定基因的差异表达和结构基因组学。

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