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首页> 美国卫生研究院文献>other >Genome-Wide Analysis of Secondary Metabolite Gene Clusters in Ophiostoma ulmi and Ophiostoma novo-ulmi Reveals a Fujikurin-Like Gene Cluster with a Putative Role in Infection
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Genome-Wide Analysis of Secondary Metabolite Gene Clusters in Ophiostoma ulmi and Ophiostoma novo-ulmi Reveals a Fujikurin-Like Gene Cluster with a Putative Role in Infection

机译：ulphiostoma ulmi和ophiostoma nov-ulmi中次生代谢产物基因簇的全基因组分析揭示了在感染中具有假定作用的Fujikurin-like基因簇

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The emergence of new microbial pathogens can result in destructive outbreaks, since their hosts have limited resistance and pathogens may be excessively aggressive. Described as the major ecological incident of the twentieth century, Dutch elm disease, caused by ascomycete fungi from the Ophiostoma genus, has caused a significant decline in elm tree populations (Ulmus sp.) in North America and Europe. Genome sequencing of the two main causative agents of Dutch elm disease (Ophiostoma ulmi and Ophiostoma novo-ulmi), along with closely related species with different lifestyles, allows for unique comparisons to be made to identify how pathogens and virulence determinants have emerged. Among several established virulence determinants, secondary metabolites (SMs) have been suggested to play significant roles during phytopathogen infection. Interestingly, the secondary metabolism of Dutch elm pathogens remains almost unexplored, and little is known about how SM biosynthetic genes are organized in these species. To better understand the metabolic potential of O. ulmi and O. novo-ulmi, we performed a deep survey and description of SM biosynthetic gene clusters (BGCs) in these species and assessed their conservation among eight species from the Ophiostomataceae family. Among 19 identified BGCs, a fujikurin-like gene cluster (OpPKS8) was unique to Dutch elm pathogens. Phylogenetic analysis revealed that orthologs for this gene cluster are widespread among phytopathogens and plant-associated fungi, suggesting that OpPKS8 may have been horizontally acquired by the Ophiostoma genus. Moreover, the detailed identification of several BGCs paves the way for future in-depth research and supports the potential impact of secondary metabolism on Ophiostoma genus’ lifestyle.
机译：新的微生物病原体的出现可能导致破坏性爆发，因为它们的宿主抵抗力有限，病原体可能具有过度侵袭性。荷兰榆树病被描述为二十世纪的主要生态事件，是由蛇纹石眼（Ophiostoma）属的子囊真菌引起的，引起了北美和欧洲榆树种群（Ulmus sp。）的显着减少。对荷兰榆树病的两种主要病原体（Ophiostoma ulmi和Ophiostoma novo-ulmi）的基因组测序以及具有不同生活方式的密切相关物种进行了独特的比较，以鉴定病原体和毒力决定因素的产生方式。在几个已确定的毒力决定因素中，已暗示次生代谢产物（SM）在植物病原体感染期间起着重要作用。有趣的是，荷兰榆树病原体的次级代谢几乎尚未被探索，关于这些物种中SM生物合成基因的组织方式知之甚少。为了更好地了解O.ulmi和O.novo-ulmi的代谢潜力，我们对这些物种中的SM生物合成基因簇（BGC）进行了深入的调查和描述，并评估了它们在眼镜蛇科（Ophiostomataceae）的8种物种中的保守性。在19个已鉴定的BGC中，一个fujikurin-like基因簇（OpPKS8）是荷兰榆树病原体特有的。系统发育分析表明，该基因簇的直系同源物在植物病原体和植物相关真菌中广泛存在，这表明OpPKS8可能已被蛇口蛇属水平地获得。此外，对几种BGC的详细鉴定为将来的深入研究铺平了道路，并支持次生代谢对Ophiostoma属的生活方式产生的潜在影响。

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