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首页> 外文期刊>Filaria journal >Cytokine profiles of filarial granulomas in jirds infected with Brugia pahangi
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Cytokine profiles of filarial granulomas in jirds infected with Brugia pahangi

机译：感染帕氏布鲁吉氏菌的蚕丝中丝状肉芽肿的细胞因子谱

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Background A granulomatous inflammatory response develops in jirds infected subcutaneously or intraperitoneally with filarial nematodes namely Brugia pahangi and B. malayi. Previous studies by light and electron microscopy have shown cellular inflammatory responses in and around these granulomas. Furthermore, the cellular inflammatory responses of granulomas found in the lymphatics and peritoneal cavity appear to be similar. The purpose of this study was to determine the cytokine profiles of granulomas in the peritoneal cavity of B. pahangi-infected jirds and to determine whether the granulomas release any proinflammatory cytokines ex vivo. Methods A semiautomated quantitative polymerase chain reaction (Q-PCR) was performed on cDNA prepared from the granulomas of infected jirds to study the species-specific mRNA expression of IL-2, IL-4, IFN-γ, IL-5, and IL-10. Genomic DNA was extracted from the granulomas, and parasite DNA was detected by Q-PCR by amplifying the HhaI repeat sequence. The levels of the inflammation-causing cytokines IL-6 and TNFα that were secreted by the granulomas were measured by cell-based assays. Results Florid granulomas showed higher levels of IFN-γ than other cytokines, linking this Th1 cytokine to the granulomatous inflammation that develops in jirds and humans. IL-4 expression was much lower than that of IFN-γ but higher than that of IL-10. A low level of IL-5 mRNA expression was detectable in all granulomas as was the level of IL-2 expression. The levels of the inflammatory cytokines IL-6 and TNFα, secreted by intact granulomas, spontaneously increased by 48 h after culture. Parasite antigen stimulation and subsequent release of IL-6 and TNFα by the granulomas indicated a moderate increase in the levels of these two cytokines. The amplification of the Brugia HhaI repeat DNA and Wolbachia 16S rDNA indicated worm components and bacterial components in the granulomatous tissue. Conclusion Granuloma development in filarial infections is a complex process involving cellular reactions responding to parasite/bacteria and their components. The interactions between worm-derived granulomas and their hosts are dynamic and multifaceted. The data collected thus far suggest that the expression profiles of many of the measured cytokines in the lymphoid tissues of Brugia-infected jirds are different from those of the cytokines in granulomas. Moreover, granulomas have the ability to secrete the inflammatory cytokines IL-6 and TNFα.
机译：背景技术肉芽肿性炎症反应在皮下或腹膜内感染丝状线虫即布鲁吉亚·彭亨（Brugia pahangi）和马来芽孢杆菌（B. malayi）的鸡中形成。先前通过光镜和电子显微镜进行的研究表明，在这些肉芽肿中和周围存在细胞炎症反应。此外，在淋巴管和腹膜腔中发现的肉芽肿的细胞炎症反应似乎相似。这项研究的目的是确定在彭亨氏芽孢杆菌感染的腹膜腔内肉芽肿的细胞因子谱，并确定肉芽肿是否在体外释放任何促炎细胞因子。方法对从感染的肠道肉芽肿制备的cDNA进行半自动定量聚合酶链反应（Q-PCR），研究IL-2，IL-4，IFN-γ，IL-5和IL的种特异性mRNA表达。 -10。从肉芽肿中提取基因组DNA，并通过扩增HhaI重复序列，通过Q-PCR检测寄生虫DNA。肉芽肿分泌的引起炎症的细胞因子IL-6和TNFα的水平通过基于细胞的测定来测量。结果小花肉芽肿显示出比其他细胞因子更高的IFN-γ水平，这将Th1细胞因子与在人和人中发展的肉芽肿性炎症联系在一起。 IL-4的表达远低于IFN-γ，但高于IL-10。在所有肉芽肿中可检测到低水平的IL-5 mRNA表达，与IL-2表达水平一样。由完整肉芽肿分泌的炎性细胞因子IL-6和TNFα的水平在培养后48小时自发增加。肉芽肿引起的寄生虫抗原刺激和随后的IL-6和TNFα释放表明这两种细胞因子的水平有所增加。 Brugia HhaI重复DNA和Wolbachia 16S rDNA的扩增表明肉芽肿组织中有蠕虫成分和细菌成分。结论丝虫感染中肉芽肿的发展是一个复杂的过程，涉及对寄生虫/细菌及其成分作出反应的细胞反应。蠕虫源性肉芽肿及其宿主之间的相互作用是动态的和多方面的。迄今为止收集的数据表明，在被布鲁贾氏菌感染的淋巴组织的淋巴组织中，许多测量的细胞因子的表达谱与肉芽肿中的细胞因子的表达谱不同。此外，肉芽肿具有分泌炎性细胞因子IL-6和TNFα的能力。

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