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首页> 外文期刊>Fisheries and Aquatic Sciences >The First Report of a Megalocytivirus Infection in Farmed Starry Flounder, Platichthys stellatus, in Korea
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The First Report of a Megalocytivirus Infection in Farmed Starry Flounder, Platichthys stellatus, in Korea

机译：在韩国养殖的比目鱼，Platiththys stellatus中，巨轮病毒感染的首次报道。

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In 2009, a systemic megalocytivirus infection associated with high mortality was detected for the first time in cultured starry flounder Platichthys stellatus in Korea. Diseased starry flounder had pale bodies and gill coloring and enlarged spleens. Histopathological examinations revealed basophilic enlarged cells in various organs of diseased starry flounder. Polymerase chain reaction (PCR) was performed on tissue samples using three published primer sets developed for the red sea bream iridovirus. PCR products were detected for all primer sets, except 1-F/1-R, which are registered by the World Organization for Animal Health (OIE). The part of the gene corresponding to the full open reading frame encoding the viral major capsid protein (MCP) was amplified by PCR. PCR products of approximately 1,581 bp were cloned, and the nucleotide sequences were analyzed phylogenetically. The MCP gene of the starry flounder iridovirus, designated SFIV0909, was identical to that of the turbot reddish body iridovirus (AB166788).
机译：2009年，在韩国养殖的比目鱼Platththys stellatus中首次检测到与高死亡率相关的系统性巨轮病毒感染。患病的星际比目鱼身体苍白，g色，脾脏肿大。组织病理学检查发现患病的比目鱼的各个器官中存在嗜碱性增大的细胞。使用针对红鲷虹膜病毒开发的三个已公开的引物组对组织样品进行聚合酶链反应（PCR）。对所有引物组（1-F / 1-R除外）的PCR产物均进行了检测，1-F / 1-R已由世界动物卫生组织（OIE）注册。通过PCR扩增与编码病毒主要衣壳蛋白（MCP）的全开放阅读框相对应的基因部分。克隆了约1,581 bp的PCR产物，并进行了系统发育分析。比目鱼比目鱼虹膜病毒的MCP基因命名为SFIV0909，与大菱形体虹膜病毒（AB166788）的MCP基因相同。

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