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首页> 外文期刊>Fisheries and Aquatic Sciences >Molecular and Histopathological Evidence of Mycobacteriosis in Paradise Fish Macropodus opercularis Imported into Korea
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Molecular and Histopathological Evidence of Mycobacteriosis in Paradise Fish Macropodus opercularis Imported into Korea

机译：进口韩国天堂鱼Macropodus opercularis分枝杆菌病的分子和组织病理学证据

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摘要

We report on mycobacteriosis in an imported tropical ornamental fish Macropodus opercularis commonly known as the paradise fish. Mass mortality occurred in paradise fish imported to Korea from Southeast Asia in 2008. The affected fish did not show any outward clinical signs, but enlargement of the spleen, kidneys, and liver was observed on dissection. Histopathological examination revealed numerous granulomas in the spleen, and acid-fast bacilli were observed in the centers of the granulomas. About 65% of spleen DNA samples were PCR positive using mycobacteria-specific primers targeting the 16S rRNA and hsp65 genes. The nucleotide identities of the 16S rRNA and hsp65 genes with those of Mycobacterium marinum were 99.5% and 99.4%, respectively. Although the bacterium was not cultured, the molecular diagnosis and histopathological findings were consistent with mycobacteriosis in paradise fish.
机译：我们报告了进口热带观赏鱼Macropodus opercularis通常被称为天堂鱼的分枝杆菌病。大规模死亡发生在2008年从东南亚进口到韩国的天堂鱼中。受感染的鱼没有显示任何向外的临床体征，但在解剖时观察到脾脏，肾脏和肝脏肿大。组织病理学检查显示在脾脏中有许多肉芽肿，在肉芽肿的中心观察到了抗酸杆菌。使用针对16S rRNA和hsp65基因的分枝杆菌特异性引物，约65％的脾脏DNA样品为PCR阳性。 16S rRNA和hsp65基因与海分枝杆菌的核苷酸同一性分别为99.5％和99.4％。尽管未培养细菌，但其分子诊断和组织病理学发现与天堂鱼中的分枝杆菌病一致。

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