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首页> 外文期刊>Fisheries and Aquatic Sciences >Establishment and Characterization of Permanent Cell Lines from Oryzias dancena Embryos
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Establishment and Characterization of Permanent Cell Lines from Oryzias dancena Embryos

机译：Oryzias dancena胚胎的永久细胞系的建立和表征

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摘要

The development of species-specific fish cell lines has become a valuable tool for biological research. In recent years, marine medaka Oryzias dancena has been recognized as a good experimental model fish but there are no reports of establishment of cell lines from this fish. In this study, two cell lines from O. dancena blastula embryos were established from 41 total trials (4.9%). The two cell lines displayed typical in vitro morphology and have been cultured for >121 passages, which corresponds to 293 days. The doubling times of the cell lines were 29.84 and 28.59 h, respectively, and both possessed the potential to expand in a clonal manner, albeit with significant differences between the two cell lines. The absence of any of the four main medium supplements; i.e., fish serum, fetal bovine serum, basic fibroblast growth factor, and medaka embryo extract, significantly inhibited growth. The proportion of cells possessing normal chromosome number was 45% and 46.7% of the cell lines, respectively. Taken together, two cell lines that proliferate continuously were established from marine medaka and these cell lines may provide a basic tool for characterizing the unique features of this fish species.
机译：物种特异性鱼类细胞系的开发已成为生物学研究的重要工具。近年来，海洋（Medaka Oryzias dancena）已被公认为是一种良好的实验模型鱼，但尚无从该鱼建立细胞系的报道。在这项研究中，从41个总试验（4.9％）中建立了来自O.dancena blastula胚芽胚胎的两个细胞系。两种细胞系均显示出典型的体外形态，并已进行了121次以上的传代培养，相当于293天。细胞系的倍增时间分别为29.84和28.59 h，尽管两种细胞系之间存在显着差异，但它们都具有以克隆方式扩增的潜力。缺少四种主要培养基补充剂中的任何一种;即，鱼血清，胎牛血清，碱性成纤维细胞生长因子和青aka胚胎提取物显着抑制了生长。具有正常染色体数目的细胞的比例分别为细胞系的45％和46.7％。两者合计，从海洋中建立了两个不断增殖的细胞系，这些细胞系可能提供表征该鱼类独特特征的基本工具。

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