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Exploiting Nanobodies in the Detection and Quantification of Human Growth Hormone via Phage-Sandwich Enzyme-Linked Immunosorbent Assay

机译:通过噬菌体-夹心酶联免疫吸附测定法开发检测和定量人类生长激素的纳米抗体

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Figure 1. Different enzyme-linked immunosorbant assay (ELISA) strategies for GH detection using specific nanobodies. (A) Schematic representation of the different methods used for detecting immobilized GH by free (I) or phage-Nb (II). Sandwich ELISA was designed for detecting GH (III and IV), phage-Nb (V), and phages (VI). Detection of phages was always performed using anti-M13-HRP antibody. (B) Detection of the immobilized (1 μg/ml) or NbGH04-captured GH (0.2 μg/ml) using five different anti-GH phage-Nb (1010 phages/ml). Significant values (*P < 0.01) comparing with their respective controls were marked with * on this graph and the following ones.
机译:图1.使用特异性纳米抗体进行GH检测的不同酶联免疫吸附测定(ELISA)策略。 (A)通过游离(I)或噬菌体-Nb(II)检测固定化GH的不同方法的示意图。夹心ELISA设计用于检测GH(III和IV),噬菌体Nb(V)和噬菌体(VI)。噬菌体的检测始终使用抗M13-HRP抗体进行。 (B)使用五种不同的抗GH噬菌体-Nb(1010噬菌体/毫升)检测固定的(1μg/ ml)或NbGH04捕获的GH(0.2μg/ ml)。与相应对照相比的显着值(* P <0.01)在此图上及以下各图中用*标记。

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