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首页> 外文期刊>Fisheries and Aquatic Sciences >The Functional Relevance of Prepro-melanin Concentrating Hormone (pMCH) to Skin Color Change, Blind-side Malpigmentation and Feeding of Oliver Flounder Paralichthys olivaceus
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The Functional Relevance of Prepro-melanin Concentrating Hormone (pMCH) to Skin Color Change, Blind-side Malpigmentation and Feeding of Oliver Flounder Paralichthys olivaceus

机译：前黑色素浓缩激素（pMCH）与肤色变化，盲侧色素沉着和Oliver Flounder Paralichthys olivaceus的喂养的功能相关性

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To assess the functional structure of prepro-melanin-concentrating hormone (pMCH), we isolated and cloned pMCH (of-pMCH) mRNA from the brain of the olive flounder, Paralichthys olivaceus, and compared its amino acid sequence with those from other animals. In addition, to examine whether activation of the brain of-pMCH gene is influenced by background color, density, and feeding, we compared pMCH mRNA activities against different background colors (bright and dark) and at different densities (100% PCA and 200% PCA). To examine whether the pMCH gene is related with malpigmentation of blind-side skin and appetite, we compared pMCH gene expression between ordinary and hypermelanic flounders, and between feeding and fasting flounders. The of-pMCH cDNA was 405 bp in the open reading frame [ORF] and encoded a protein of 135 amino acids; MCH was 51 bp in length and encoded a protein of 17 amino acids. An obvious single band of the expected size was obtained from the brain and pituitary by RT-PCR. In addition, of-pMCH gene activity was significantly higher in the bright background only at low density ( 200% PCA), and in hypermelano fish. These results suggest that skin whitening camouflage of the flounder is induced by high MCH gene activity, and the density disturbs the function of background color in the physiological color change. Moreover, our data suggest that a low level of MCH gene activity may be related to malpigmentation of the blind-side skin. In feeding, although pMCH gene activity was significantly increased by feeding in the white background, the pMCH gene activity in the dark background was not influenced by feeding, indicating that the MCH gene activity increased by feeding can be offset by dark background color, or is unaffected by appetite. In conclusion, this study showed that MCH gene expression is related to ocular-skin whitening camouflage and blind-skin hypermelanosis, and is influenced by background color and density.
机译：为了评估黑色素前浓缩激素（pMCH）的功能结构，我们从橄榄比目鱼Paralichthys olivaceus的大脑中分离并克隆了pMCH（of-pMCH）mRNA，并将其氨基酸序列与其他动物的氨基酸序列进行了比较。此外，为了检查pMCH基因的大脑激活是否受背景颜色，密度和进食的影响，我们比较了pMCH mRNA针对不同背景颜色（亮和暗）和不同密度（100％PCA和200％）的活性。 PCA）。为了检查pMCH基因是否与盲侧皮肤和食欲不振相关，我们比较了普通和高黑度比目鱼之间以及进食和禁食比目鱼之间的pMCH基因表达。 -pMCH cDNA的开放阅读框[ORF]为405 bp，编码135个氨基酸。 MCH的长度为51 bp，编码17个氨基酸的蛋白质。通过RT-PCR从大脑和垂体获得了预期大小的明显单条带。此外，仅在低密度（200％PCA）时，在明亮的背景中以及在超黑素鱼类中，of-pMCH基因活性显着较高。这些结果表明，比目鱼的皮肤增白伪装是由高MCH基因活性引起的，并且密度扰乱了背景色在生理颜色变化中的功能。此外，我们的数据表明，低水平的MCH基因活性可能与盲侧皮肤的病变程度有关。在饲养中，尽管通过饲喂白色背景可显着提高pMCH基因活性，但在黑暗背景下的pMCH基因活性不受饲喂的影响，这表明通过饲喂而增加的MCH基因活性可被深色背景色抵消，或不受食欲的影响。总之，这项研究表明，MCH基因的表达与皮肤增白伪装和皮肤盲变黑有关，并且受背景颜色和密度的影响。

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