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首页> 外文期刊>Fisheries and Aquatic Sciences >Comparison of Free and Dipeptide Lysine Utilization in Diets for Juvenile Olive Flounder Paralichthys olivaceus
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Comparison of Free and Dipeptide Lysine Utilization in Diets for Juvenile Olive Flounder Paralichthys olivaceus

机译：幼稚比目鱼Olivaceus饮食中游离和二肽赖氨酸利用的比较

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摘要

We compared the utilization efficiency of free lysine (FL) and dipeptide lysine-glycine (LG) in terms of growth performance and whole-body amino acid composition in olive flounder Paralichthys olivaceus. A basal experimental diet was formulated to contain 0.5% (basal) lysine from fish meal, and four other diets were prepared by supplementing 0.5% or 1.0% of either FL or LG. The experiment was performed in triplicate, and 20 randomly selected fish averaging were fed one of the test diets at the rate of 3% BW/day twice daily for 6 weeks. At the end of the feeding trial, fish fed the basal diet showed significantly less weight gain than did the other groups. The results of a two-way ANOVA showed that both lysine level (P = 0.001) and type (P = 0.034) influenced growth rate; however, we found no significant interaction between lysine level and form (P > 0.05). Our results revealed a significant improvement in protein efficiency ratio (PER) with each increment of dietary lysine, and the groups fed LG-supplemented diets showed higher PER than did those offered FL. Hepatosomatic and viscerosomatic indices were significantly influenced by lysine level and form, and higher values were recorded in fish fed diets containing LG. Significantly higher whole-body arginine levels were found in LG-fed groups, and a significant interaction was observed between lysine level and form (P = 0.009). Whole-body valine and aspartic acid contents were affected by lysine level, and alanine concentration was influenced by both lysine level and form. Our findings indicate that juvenile olive flounder can utilize LG more efficiently than FL for protein synthesis.
机译：我们根据橄榄比目鱼Paralichthys olivaceus的生长性能和全身氨基酸组成比较了游离赖氨酸（FL）和二肽赖氨酸甘氨酸（LG）的利用效率。基本实验饮食的配方中鱼粉中的赖氨酸含量为0.5％，其他四种饮食通过添加0.5％或1.0％的FL或LG来制备。实验一式三份进行，平均每天随机选择20条鱼，以3％BW /天的速率喂食其中一种测试日粮，每天两次，持续6周。在饲喂试验结束时，饲喂基础饮食的鱼体重增加明显少于其他组。双向方差分析的结果表明，赖氨酸水平（P = 0.001）和类型（P = 0.034）都影响生长速度;然而，我们发现赖氨酸水平和形式之间没有显着的相互作用（P> 0.05）。我们的研究结果表明，随着日粮赖氨酸含量的增加，蛋白质效率比（PER）显着提高，并且LG添加日粮的组显示的PER高于FL组。肝素和内脏指数受赖氨酸水平和形式的显着影响，在含LG的鱼饲料中记录到更高的值。在LG喂养的组中发现整体精氨酸水平显着提高，并且赖氨酸水平和形式之间存在显着的相互作用（P = 0.009）。赖氨酸水平影响全身缬氨酸和天冬氨酸的含量，赖氨酸水平和形式都影响丙氨酸的浓度。我们的发现表明，少年比目鱼可以比LG更有效地利用LG进行蛋白质合成。

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