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首页> 外文期刊>Fisheries and Aquatic Sciences >Genomic Organization and Isoform-Dependent Expression Patterns of Wap65 genes in Various Tissues during Immune Challenges in the Mud Loach Misgurnus mizolepis
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Genomic Organization and Isoform-Dependent Expression Patterns of Wap65 genes in Various Tissues during Immune Challenges in the Mud Loach Misgurnus mizolepis

机译：泥LoMisgurnus mizolepis免疫攻击期间各种组织中Wap65基因的基因组组织和同工型依赖性表达模式

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摘要

Genomic organization, including the structural characteristics of 5'-flanking regions of two 65-kDa protein (WAP65) isoform genes associated with warm temperature acclimation, were characterized and their transcriptional responses to immune challenges were examined in the intestine, kidney and spleen of the mud loach (Misgurnus mizolepis; Cypriniformes). Both mud loach Wap65 isoform genes displayed a 10-exon structure that is common to most teleostean Wap65 genes. The two mud loach Wap65 isoforms were predicted to possess various stress- and immune-related transcription factor binding sites in their regulatory regions; however, the predicted motif profiles differed between the two isoforms, and the inflammation-related transcription factor binding motifs, such as NF- and CREBP sites, were more highlighted in the Wap65-2 isoform than the Wap65-1 isoform. The results of qRT-PCR indicated that experimental immune challenges using Edwardsiella tarda, lipopolysaccharide or polyI:C induced the Wap65-2 isoform more than Wap65-1 isoform, although modulation patterns in response to these challenges were tissue- and stimulant-dependent. This study confirms that functional diversification between the two mud loach Wap65 isoforms (i.e., closer involvement of Wap65-2 in the acute phase of inflammation and innate immunity) occurs at the mRNA level in multiple tissues, and suggests that such differential modulation patterns between the two isoforms are related to the different transcription factor binding profiles in their regulatory regions.
机译：基因组的组织，包括两个65 kDa蛋白（WAP65）与温暖的温度适应相关的同工型基因的5'侧翼区域的结构特征，并在肠道，肾脏和脾脏中检查了它们对免疫挑战的转录反应。泥lo（Misgurnus mizolepis;楔形目）。两种泥lo Wap65同工型基因都显示出10外显子结构，这是大多数硬骨Wap65基因共有的结构。两种泥loWap65同工型据预测在其调控区具有各种应激和免疫相关的转录因子结合位点。但是，两种亚型之间预测的基序谱不同，并且与Wap65-1亚型相比，Wap65-2亚型中炎症相关转录因子结合基序（如NF-和CREBP位点）更加突出。 qRT-PCR的结果表明，使用爱德华氏菌，脂多糖或polyI：C进行的实验性免疫激发比Wap65-1异构体更能诱导Wap65-2异构体，尽管响应于这些挑战的调节模式是组织和兴奋剂依赖性的。这项研究证实了两种泥lo Wap65亚型之间的功能多样化（即Wap65-2在炎症和先天免疫的急性期更紧密地参与）发生在多个组织的mRNA水平上，并且表明这种不同的调制方式之间存在差异。两个同工型与它们调节区域中不同的转录因子结合谱有关。

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