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首页> 外文期刊>Fisheries and Aquatic Sciences >Molecular Characterization and Expression Pattern of Na+-K+-2Cl- Cotransporter 2 (NKCC2) in the Intestine of Starry Flounder Platichthys stellatus after Bacterial Challenge
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Molecular Characterization and Expression Pattern of Na+-K+-2Cl- Cotransporter 2 (NKCC2) in the Intestine of Starry Flounder Platichthys stellatus after Bacterial Challenge

机译：Na + -K + -2Cl - Cotransporter 2（NKCC2）在细菌后的比目鱼Plateththys stellatus肠中的分子表征和表达模式挑战

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We identified the cotransporter 2 (NKCC2) cDNA isoform from starry flounder, Platichthys stellate. The NKCC2 cDNA encoded a polypeptide of 1,043 amino acids representing 12 putative transmembrane domains based on the bioinformatic topology prediction. In addition, starry flounder NKCC2 possessed highly conserved residues within transmembrane domain 4, known as an essential site for its function. End-point reverse transcription-polymerase chain reaction analysis revealed that the NKCC2 transcript was moderately expressed only in the anterior and posterior intestines and the rectum. The NKCC2 mRNA level in the rectum, but not in other segments, was significantly induced 3 days post Streptococcus parauberis challenge, indicating that excess salt may be transported into the rectum. Taken together, our data indicate that an S. parauberis infection could tip the intestinal fluid balance in favor of fluid accumulation, indicating that bacterial pathogens can interfere with intestinal osmotic balance and normal mucosal immune homeostasis.
机译：我们从繁重的比目鱼，Platiththyss星状鉴定了cotransporter 2（NKCC2）cDNA亚型。 NKCC2 cDNA基于生物信息拓扑预测，编码了1,043个氨基酸的多肽，代表12个推定的跨膜结构域。另外，比目鱼NKCC2在跨膜结构域4内具有高度保守的残基，被称为其功能的重要位点。端点逆转录-聚合酶链反应分析表明，NKCC2转录本仅在前，后肠和直肠中表达。副链球菌攻击3天后，直肠中NKCC2 mRNA水平显着升高，而其他部分则未明显升高，这表明过量的盐可能被转运到直肠中。两者合计，我们的数据表明，副乳房链球菌感染可以使肠道液平衡趋于有利于液体积聚，表明细菌性病原体可以干扰肠道渗透平衡和正常的粘膜免疫稳态。

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