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首页> 外文期刊>Frontiers in Microbiology >Characterization and Strain Improvement of a Hypercellulytic Variant, Trichoderma reesei SN1, by Genetic Engineering for Optimized Cellulase Production in Biomass Conversion Improvement
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Characterization and Strain Improvement of a Hypercellulytic Variant, Trichoderma reesei SN1, by Genetic Engineering for Optimized Cellulase Production in Biomass Conversion Improvement

机译：遗传工程改造纤维素酶变种里氏木霉 SN1的表征和菌株改良，以优化生物质转化中的纤维素酶生产

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摘要

The filamentous fungus Trichoderma reesei is a widely used strain for cellulolytic enzyme production. A hypercellulolytic T. reesei variant SN1 was identified in this study and found to be different from the well-known cellulase producers QM9414 and RUT-C30. The cellulose-degrading enzymes of T. reesei SN1 show higher endoglucanase (EG) activity but lower β-glucosidase (BGL) activity than those of the others. A uracil auxotroph strain, SP4, was constructed by pyr4 deletion in SN1 to improve transformation efficiency. The BGL1-encoding gene bgl1 under the control of a modified cbh1 promoter was overexpressed in SP4. A transformant, SPB2, with four additional copies of bgl1 exhibited a 17.1-fold increase in BGL activity and a 30.0% increase in filter paper activity. Saccharification of corncob residues with crude enzyme showed that the glucose yield of SPB2 is 65.0% higher than that of SP4. These results reveal the feasibility of strain improvement through the development of an efficient genetic transformation platform to construct a balanced cellulase system for biomass conversion.
机译：丝状真菌里氏木霉是用于纤维素分解酶生产的广泛使用的菌株。在这项研究中鉴定出一种超纤维素分解里氏木霉变种SN1，发现它与著名的纤维素酶生产商QM9414和RUT-C30不同。里氏木霉SN1的纤维素降解酶显示出比其他酶更高的内切葡聚糖酶（EG）活性，但更低的β-葡萄糖苷酶（BGL）活性。通过在SN1中删除pyr4来构建尿嘧啶营养缺陷型菌株SP4，以提高转化效率。在修饰的cbh1启动子的控制下，BGL1编码基因bgl1在SP4中过表达。带有四个额外bgl1拷贝的转化子SPB2的BGL活性提高了17.1倍，滤纸活性提高了30.0％。用粗酶糖化玉米芯残留物表明，SPB2的葡萄糖产率比SP4高65.0％。这些结果揭示了通过开发有效的遗传转化平台以构建用于生物质转化的平衡纤维素酶系统的菌株改良的可行性。

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