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首页> 外文期刊>Journal of King Saud University >Optimization and strain improvement by mutation for enhanced cellulase production by Bacillus sp. (MTCC10046) isolated from cow dung
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Optimization and strain improvement by mutation for enhanced cellulase production by Bacillus sp. (MTCC10046) isolated from cow dung

机译：通过突变优化和菌株改良，以增强芽孢杆菌 sp的纤维素酶生产。 （MTCC10046）从牛粪中分离

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摘要

The goal was to investigate the cellulase enzyme production ability of bacterial strain C1 isolated from cow dung and identified as Bacillus sp. on the basis of 16 S rDNA sequence homology. The effects of different carbon sources like Carboxymethyl cellulose (CMC), avicel, starch, maltose, sucrose, glucose, fructose, galactose and lactose on cellulase production at varying environmental parameters of incubation period (2–10 days), temperature (35–55 °C), and pH (6.0–8.5) were examined. The CMC was the best carbon source for cellulase production followed by lactose in this bacterial strain. The maximum enzyme production was achieved at a temperature of 50 °C by Bacillus sp. with pH of 7.0 on the 8th day of growth. The nitrogen source NH 4 NO 3 at 0.175% was optimum for cellulase production by this bacterium. A putative mutant (C1M 26 ) was screened from wild C1 strain after mutagenesis with N-methyl-N′-nitro-N-nitrosoguanidine (NTG) as a mutagenic agent. The mutant C1M 26 produced a larger amount of cellulase in comparison to wild type C1 strain.
机译：目的是研究分离自牛粪并鉴定为芽孢杆菌（Bacillus sp）的细菌菌株C1的纤维素酶生产能力。基于16 S rDNA序列同源性。在不同的孵育时间（2-10天），环境温度（35-55）环境参数下，羧甲基纤维素（CMC），avicel，淀粉，麦芽糖，蔗糖，葡萄糖，果糖，半乳糖和乳糖等不同碳源对纤维素酶生产的影响°C）和pH（6.0-8.5）进行了检查。 CMC是产生纤维素酶的最佳碳源，其次是该菌株中的乳糖。 Bacillus sp。在50°C的温度下获得了最大的酶产量。生长第8天的pH值为7.0。 0.175％的氮源NH 4 NO 3最适合该细菌生产纤维素酶。使用N-甲基-N'-硝基-N-亚硝基胍（NTG）作为诱变剂诱变后，从野生C1菌株中筛选出推定的突变体（C1M 26）。与野生型C1菌株相比，突变体C1M 26产生大量的纤维素酶。

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