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首页> 外文期刊>Frontiers in Immunology >M1 and M2 Monocytes in Rheumatoid Arthritis: A Contribution of Imbalance of M1/M2 Monocytes to Osteoclastogenesis
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M1 and M2 Monocytes in Rheumatoid Arthritis: A Contribution of Imbalance of M1/M2 Monocytes to Osteoclastogenesis

机译：类风湿关节炎中的M1和M2单核细胞：M1 / M2单核细胞对破骨细胞形成的不平衡贡献

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Objectives We investigated the relationships among M1 monocytes, M2 monocytes, osteoclast (OC) differentiation ability, and clinical characteristics in patients with rheumatoid arthritis (RA). Methods Peripheral blood mononuclear cells (PBMCs) were isolated from RA patients and healthy donors, and we then investigated the number of M1 monocytes or M2 monocytes by fluorescence-activated cell sorting. We also obtained and cultured CD14-positive cells from PBMCs from RA patients and healthy donors to investigate OC differentiation in vitro . Results Forty RA patients and 20 healthy donors were included. Twenty-two patients (55%) were anticitrullinated protein antibody (ACPA) positive. The median M1/M2 ratio was 0.59 (0.31–1.11, interquartile range). There were no significant differences between the RA patients and healthy donors. There was a positive correlation between the M1/M2 ratio and the differentiated OC number in vitro in RA patients (ρ?=?0.81, p ?1 (having relatively more M1 monocytes) had higher C-reactive protein and erythrocyte sedimentation rates than RA patients with M1/M2 ratios ≤1. M1-dominant monocytes in vitro produced higher concentrations of interleukin-6 upon stimulation with lipopolysaccharide than M2 monocytes. Conclusion M1/M2 monocytes imbalance strongly contributes to osteoclastogenesis of RA patients. Our findings cast M1 and M2 monocyte subsets in a new light as a new target of treatments for RA to prevent progression of osteoclastic bone destruction.
机译：目的我们研究类风湿关节炎（RA）患者的M1单核细胞，M2单核细胞，破骨细胞（OC）分化能力和临床特征之间的关系。方法从RA患者和健康捐献者中分离外周血单个核细胞（PBMC），然后通过荧光激活细胞分选法研究M1单核细胞或M2单核细胞的数目。我们还从RA患者和健康供体的PBMC中获得并培养了CD14阳性细胞，以研究OC的体外分化。结果包括40名RA患者和20名健康捐献者。 22名患者（55％）的抗瓜氨酸化蛋白抗体（ACPA）阳性。中位数M1 / M2比率为0.59（0.31-1.11，四分位间距）。 RA患者与健康捐献者之间无显着差异。 RA患者的M1 / M2比值与体外分化的OC值呈正相关（ρ= 0.81，p <0.001）。与ACPA阴性的患者相比，ACPA阳性的患者体内M1 / M2比率显着更高（p = 0.0028），体外OC的数量也明显较多（p = 0.005）。多变量回归分析显示，M1 / M2比是体外破骨细胞形成的唯一重要因素。 M1 / M2比率> 1（具有相对更多的M1单核细胞）的RA患者比M1 / M2比率≤1的RA患者具有更高的C反应蛋白和红细胞沉降率。脂多糖刺激后，M1优势单核细胞在体外产生的浓度高于M2单核细胞。结论M1 / M2单核细胞失衡是导致RA患者破骨细胞形成的重要原因。我们的发现以新的视角投射了M1和M2单核细胞亚群，作为RA预防破骨细胞破坏的新方法。

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