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首页> 外文期刊>Frontiers in Plant Science >Comprehensive Transcriptome Profiling Reveals Long Noncoding RNA Expression and Alternative Splicing Regulation during Fruit Development and Ripening in Kiwifruit ( Actinidia chinensis)
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Comprehensive Transcriptome Profiling Reveals Long Noncoding RNA Expression and Alternative Splicing Regulation during Fruit Development and Ripening in Kiwifruit ( Actinidia chinensis)

机译：完整的转录组分析揭示了猕猴桃果实发育和成熟过程中的长非编码RNA表达和可变剪接调控。（中华猕猴桃）

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Genomic and transcriptomic data on kiwifruit ( Actinidia chinensis ) in public databases are very limited despite its nutritional and economic value. Previously, we have constructed and sequenced nine fruit RNA-Seq libraries of A. chinensis “Hongyang” at immature, mature, and postharvest ripening stages of fruit development, and generated over 66.2 million paired-end and 24.4 million single-end reads. From this dataset, here we have identified 7051 long noncoding RNAs (lncRNAs), 29,327 alternative splicing (AS) events and 2980 novel protein-coding genes that were not annotated in the draft genome of “Hongyang.” AS events were demonstrated in genes involved in the synthesis of nutritional metabolites in fruit, such as ascorbic acids, carotenoids, anthocyanins, and chlorophylls, and also in genes in the ethylene signaling pathway, which plays an indispensable role in fruit ripening. Additionally, transcriptome profiles and the contents of sugars, organic and main amino acids were compared between immature, mature, and postharvest ripening stages in kiwifruits. A total of 5931 differentially expressed genes were identified, including those associated with the metabolism of sugar, organic acid, and main amino acids. The data generated in this study provide a foundation for further studies of fruit development and ripening in kiwifruit, and identify candidate genes and regulatory elements that could serve as targets for improving important agronomic traits through marker assisted breeding and biotechnology.
机译：尽管具有营养和经济价值，但公共数据库中有关猕猴桃（猕猴桃）的基因组和转录组数据非常有限。以前，我们已经在水果发育的未成熟，成熟和收获后成熟阶段构建并测序了九个中国宏草“洪阳”的水果RNA-Seq文库，并产生了超过6,620万对的配对末端和2,440万个单末端读取。从该数据集中，我们确定了7051个长非编码RNA（lncRNA），29,327个替代剪接（AS）事件和2980个未在“鸿阳”基因组草图中注释的新型蛋白质编码基因。 AS事件在涉及水果中营养代谢产物合成的基因（例如抗坏血酸，类胡萝卜素，花色苷和叶绿素）中以及在乙烯信号传导途径的基因中得到证明，乙烯信号途径在果实成熟中起着不可或缺的作用。此外，比较了猕猴桃未成熟，成熟和收获后成熟阶段的转录组概况以及糖，有机和主要氨基酸的含量。共鉴定出5931个差异表达基因，包括与糖，有机酸和主要氨基酸代谢相关的基因。该研究产生的数据为进一步研究猕猴桃的果实发育和成熟提供了基础，并确定了候选基因和调控元件，这些基因和调控元件可以作为通过标记辅助育种和生物技术改善重要农艺性状的目标。

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