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首页> 外文期刊>Frontiers in Plant Science >Overexpression of Nictaba-Like Lectin Genes from Glycine max Confers Tolerance toward Pseudomonas syringae Infection, Aphid Infestation and Salt Stress in Transgenic Arabidopsis Plants
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Overexpression of Nictaba-Like Lectin Genes from Glycine max Confers Tolerance toward Pseudomonas syringae Infection, Aphid Infestation and Salt Stress in Transgenic Arabidopsis Plants

机译：来自 Glycine max 的 Nictaba -类凝集素基因的过表达赋予对转基因拟南芥的丁香假单胞菌感染，蚜虫侵染和盐胁迫的耐受性/ italic>植物

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Plants have evolved a sophisticated immune system that allows them to recognize invading pathogens by specialized receptors. Carbohydrate-binding proteins or lectins are part of this immune system and especially the lectins that reside in the nucleocytoplasmic compartment are known to be implicated in biotic and abiotic stress responses. The class of Nictaba-like lectins (NLL) groups all proteins with homology to the tobacco ( Nicotiana tabacum ) lectin, known as a stress-inducible lectin. Here we focus on two Nictaba homologs from soybean ( Glycine max ), referred to as Gm NLL1 and Gm NLL2. Confocal laser scanning microscopy of fusion constructs with the green fluorescent protein either transiently expressed in Nicotiana benthamiana leaves or stably transformed in tobacco BY-2 suspension cells revealed a nucleocytoplasmic localization for the Gm NLLs under study. RT-qPCR analysis of the transcript levels for the Nictaba-like lectins in soybean demonstrated that the genes are expressed in several tissues throughout the development of the plant. Furthermore, it was shown that salt treatment, Phytophthora sojae infection and Aphis glycines infestation trigger the expression of particular NLL genes. Stress experiments with Arabidopsis lines overexpressing the NLLs from soybean yielded an enhanced tolerance of the plant toward bacterial infection ( Pseudomonas syringae ), insect infestation ( Myzus persicae ) and salinity. Our data showed a better performance of the transgenic lines compared to wild type plants, indicating that the NLLs from soybean are implicated in the stress response. These data can help to further elucidate the physiological importance of the Nictaba-like lectins from soybean, which can ultimately lead to the design of crop plants with a better tolerance to changing environmental conditions.
机译：植物已经进化出一种先进的免疫系统，可以使它们通过专门的受体识别入侵的病原体。碳水化合物结合蛋白或凝集素是该免疫系统的一部分，尤其是驻留在核质区室中的凝集素与生物和非生物应激反应有关。一类类似于烟草的凝集素（NLL）将所有与烟草（Nicotiana tabacum）凝集素具有同源性的蛋白质归为一组，这种蛋白质被称为胁迫诱导型凝集素。在这里，我们重点研究大豆（Glycine max）的两个Nictaba同源物，分别称为Gm NLL1和Gm NLL2。共聚焦激光扫描显微镜观察融合结构与绿色荧光蛋白的融合结构，该蛋白在烟叶中瞬时表达或在烟草BY-2悬浮细胞中稳定转化，揭示了正在研究的Gm NLL的胞质定位。 RT-qPCR分析大豆中Nictaba样凝集素的转录水平表明，在植物的整个发育过程中，基因在多个组织中表达。此外，研究表明，盐处理，大豆疫霉菌感染和蚜虫甘氨酸侵扰会触发特定NLL基因的表达。拟南芥品系过表达大豆的NLLs的逆境实验提高了植物对细菌感染（Pseudomonas syringae），昆虫侵染（Myzus persicae）和盐度的耐受性。我们的数据显示，与野生型植物相比，转基因品系的性能更好，表明大豆的NLL与胁迫反应有关。这些数据可以帮助进一步阐明大豆中类似尼古巴的凝集素的生理重要性，这最终可以导致设计出对环境条件有更好耐受性的农作物。

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