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首页> 外文期刊>Frontiers in Plant Science >The Latex Protein MLX56 from Mulberry ( Morus multicaulis) Protects Plants against Insect Pests and Pathogens
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The Latex Protein MLX56 from Mulberry ( Morus multicaulis) Protects Plants against Insect Pests and Pathogens

机译：桑树中的乳胶蛋白MLX56（桑属）保护植物免受害虫和病原体的侵害

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摘要

Biotic stresses are major constraints limiting the leaf quality and productivity of mulberry. MLX56 is a unique chitin-binding protein isolated from Shin-Ichinose ( Morus alba ) latex that displays toxicity against lepidopteran caterpillars. In this study, the full-length cDNA encoding MLX56 was isolated from Husang 32 ( M. multicaulis ) and designated HMLX56 . Amino acid sequence analysis and protein modeling of three MLX56 proteins showed that they were highly conserved among Morus species. Tissue expression pattern analysis showed that the HMLX56 gene was strongly expressed in mulberry bark and leaves but only slightly expressed in fruits. In addition, analysis of GUS expression indicated that the promoter of HMLX56 showed higher transcriptional activity along the vascular strands, and its activity can be regulated by various environmental factors. Like the MLX56 protein from M. alba , the HMLX56 protein showed toxicity to Plutella xylostella . Moreover, when the HMLX56 gene was ectopically expressed in Arabidopsis, the transgenic plants showed enhanced resistance to aphids, the fungal pathogen Botrytis cinerea and the bacterial pathogen Pseudomonas syringae pv. tomato DC3000. Our data suggest that the HMLX56 protein has a lectin-like molecular structure consisting of two hevein-like chitin-binding domains which provide not only chitin-binding activities but also other mechanisms of defense. The information provided here improves our understanding of the potential functions and defense mechanisms of MLX56 proteins, enabling in-depth functional analysis of latex exudates and perhaps facilitating mulberry genetic improvement in the future.
机译：生物胁迫是限制桑叶质量和生产力的主要限制因素。 MLX56是一种分离自Shin-Ichinose（Morus alba）乳胶的独特的几丁质结合蛋白，对鳞翅目毛毛虫具有毒性。在这项研究中，从虎山32（M. multicaulis）中分离出编码MLX56的全长cDNA，并将其命名为HMLX56。三种MLX56蛋白质的氨基酸序列分析和蛋白质建模表明，它们在桑属物种中高度保守。组织表达模式分析表明，HMLX56基因在桑树的树皮和叶子中强烈表达，而在果实中仅少量表达。另外，对GUS表达的分析表明，HMLX56的启动子沿血管链显示出更高的转录活性，并且其活性可以受多种环境因素调节。像白念珠菌的MLX56蛋白一样，HMLX56蛋白对小菜蛾也表现出毒性。此外，当HMLX56基因在拟南芥中异位表达时，转基因植物对蚜虫，真菌病原体灰葡萄孢和细菌病原体丁香假单胞菌pv的抗性增强。番茄DC3000。我们的数据表明，HMLX56蛋白具有类似于凝集素的分子结构，该结构由两个橡胶素样几丁质结合域组成，不仅提供了几丁质结合活性，还提供了其他防御机制。此处提供的信息有助于我们了解MLX56蛋白的潜在功能和防御机制，从而能够对乳胶渗出液进行深入的功能分析，并可能在将来促进桑树的遗传改良。

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