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Influence of in vitro maturation duration on embryo yield in goats

机译:体外成熟时间对山羊胚胎产量的影响

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Oocytes retrieved from goat ovaries are usually still in immature stage and thus, are not ready for fertlisation. Hence, the oocytes need to be cultured in maturation medium for certain duration before they fertilise. In this experiment, the effect of in vitro maturation duration on laparoscopic oocyte pick-up (LOPU)-derived caprine oocytes towards embryo yield (4-cell stage taken as the minimum criterion) was investigated. Separate batches of oocytes were matured for 18 to 20 h, 21 to 23 h, 24 to 26 h and 27 to 29 h, respectively, at 38.5°C under 5% CO2 in humidified air before they were in vitro fertilised and cultured. LOPU-derived oocytes matured for 18 to 20 h improved (p<0.01) embryo yield (42.6%). In conclusion, appropriate IVM duration will improve the in vitro fertilsation performance in goats.
机译:从山羊卵巢中取出的卵母细胞通常仍处于未成熟阶段,因此尚未准备好受精。因此,卵母细胞在受精之前需要在成熟培养基中培养一定的时间。在此实验中,研究了体外成熟持续时间对腹腔镜卵母细胞拾取(LOPU)衍生的山羊卵母细胞对胚胎产量(以4细胞阶段为最低标准)的影响。分批卵母细胞分别在38.5°C,5%的CO2和湿空气中分别于38.5°C熟化18至20小时,21至23小时,24至26小时和27至29小时,然后进行体外受精和培养。 LOPU衍生的卵母细胞成熟18至20 h可提高(p <0.01)胚胎产量(42.6%)。总之,适当的IVM持续时间将改善山羊的体外受精性能。

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