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首页> 外文期刊>Marine Drugs >Characterization of MK 8 (H 2 ) from Rhodococcus sp. B7740 and Its Potential Antiglycation Capacity Measurements
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Characterization of MK 8 (H 2 ) from Rhodococcus sp. B7740 and Its Potential Antiglycation Capacity Measurements

机译：Rhodococcus sp。的MK 8（H 2）的表征。 B7740及其潜在的抗糖化能力测量

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摘要

Menaquinone (MK) has an important role in human metabolism as an essential vitamin (VK 2 ), which is mainly produced through the fermentation of microorganisms. MK 8 (H 2 ) was identified to be the main menaquinone from Rhodococcus sp. B7740, a bacterium isolated from the arctic ocean. In this work, MK 8 (H 2 ) (purity: 99.75%) was collected through a convenient and economic extraction process followed by high-speed countercurrent chromatography (HSCCC) purification. Additionally, high-resolution mass spectrometry (HRMS) was performed for further identification and the hydrogenation position of MK 8 (H 2 ) (terminal unit) was determined using nuclear magnetic resonance (NMR) for the first time. MK 8 (H 2 ) showed a superior antioxidant effect and antiglycation capacity compared with ubiquinone Q10 and MK 4 . High-performance liquid chromatography–mass spectrometer (HPLC-MS/MS) and molecular docking showed the fine interaction between MK 8 (H 2 ) with methylglyoxal (MGO) and bull serum albumin (BSA), respectively. These properties make MK 8 (H 2 ) a promising natural active ingredient with future food and medicine applications.
机译：甲萘醌（MK）在人体新陈代谢中作为必需的维生素（VK 2）具有重要作用，维生素主要通过微生物的发酵产生。 MK 8（H 2）被鉴定为来自红球菌属的主要甲萘醌。 B7740，一种从北冰洋分离的细菌。在这项工作中，MK 8（H 2）（纯度：99.75％）是通过一种方便且经济的萃取方法进行收集的，然后进行高速逆流色谱（HSCCC）纯化。此外，还进行了高分辨率质谱（HRMS）的进一步鉴定，并首次使用核磁共振（NMR）确定了MK 8（H 2）（末端单元）的氢化位置。与泛醌Q10和MK 4相比，MK 8（H 2）具有更好的抗氧化作用和抗糖化能力。高效液相色谱-质谱仪（HPLC-MS / MS）和分子对接分别显示MK 8（H 2）与甲基乙二醛（MGO）和牛血清白蛋白（BSA）之间的良好相互作用。这些特性使MK 8（H 2）在未来的食品和药品应用中成为有前途的天然活性成分。

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