...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Mass Spectrometry Letters >Loss of Potential Biomarker Proteins Associated with Abundant Proteins during Abundant Protein Removal in Sample Pretreatment
【24h】

Loss of Potential Biomarker Proteins Associated with Abundant Proteins during Abundant Protein Removal in Sample Pretreatment

机译：样品预处理中大量蛋白质的去除过程中与大量蛋白质相关的潜在生物标志物蛋白质的损失

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Capture of non-glycoproteins during lectin affinity chromatography is frequently observed, although it would seem to be anomalous. In actuality, lectin affinity chromatography works at post-translational modification (PTM) sites on a glycoprotein which is not involved in protein-protein interactions (PPIs). In this study, serial affinity column set (SACS) using lectins followed by proteomics methods was used to identify PPI mechanisms of captured proteins in human plasma. MetaCore, STRING, Ingenuity Pathway Analysis (IPA), and IntAct were individually used to elucidate the interactions of the identified abundant proteins and to obtain the corresponding interaction maps. The abundant non-glycoproteins were captured with the binding to the selected glycoproteins. Therefore, depletion process in sample pretreatment for abundant protein removal should be considered with more caution because it may lose precious disease-related low abundant proteins through PPIs of the removed abundant proteins in human plasma during the depletion process in biomarker discovery. Glycoproteins bearing specific glycans are frequently associated with cancer and can be specifically isolated by lectin affinity chromatography. Therefore, SACS using Lycopersicon esculentum lectin (LEL) can also be used to study disease interactomes.
机译：尽管似乎是异常的，但经常在凝集素亲和层析过程中捕获非糖蛋白。实际上，凝集素亲和色谱在糖蛋白上的翻译后修饰（PTM）位点起作用，该位点不参与蛋白-蛋白相互作用（PPI）。在这项研究中，使用凝集素和蛋白质组学方法的串行亲和柱集（SACS）用于鉴定人血浆中捕获蛋白的PPI机制。 MetaCore，STRING，机能途径分析（IPA）和IntAct分别用于阐明鉴定出的丰富蛋白质的相互作用并获得相应的相互作用图。捕获的大量非糖蛋白与所选糖蛋白结合。因此，应谨慎考虑样品预处理中用于去除大量蛋白质的消耗过程，因为在生物标志物发现过程中，通过血浆中去除的丰富蛋白质的PPI可能会丢失与疾病相关的珍贵疾病相关的低含量蛋白质。带有特定聚糖的糖蛋白通常与癌症有关，可以通过凝集素亲和色谱法进行特异性分离。因此，使用番茄番茄凝集素（LEL）的SACS也可以用于研究疾病的相互作用组。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号