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首页> 外文期刊>Mass Spectrometry Letters >Evaluation of MALDI Biotyping for Rapid Subspecies Identification of Carbapenemase-Producing Bacteria via Protein Profiling
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Evaluation of MALDI Biotyping for Rapid Subspecies Identification of Carbapenemase-Producing Bacteria via Protein Profiling

机译：MALDI生物分型技术通过蛋白质谱分析快速鉴定产碳青霉烯酶的细菌的亚型评估。

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The method of direct mass spectrometry profiling is reliable and reproducible for the rapid identification of clinical isolates of bacteria and fungi. This is the first study evaluating the approach of MALDI-TOF mass spectrometry profiling for rapid identification of carbapenemase-resistant enterobacteriaceae (CRE). Proof of concept was achieved by the discrimination of CRE using MALDI Biotyper MS based on the protein. This profiling appears promising by the visual observation of consistent unique peaks, albeit low intensity, that could be picked up from the mean spectra (MSP) method. The Biotyper MSP creation and identification methods needed to be optimized to provide significantly improved differences in scores to allow for subspecies identification with and without carbapenemases. These spectra were subjected to visual peak picking and in all cases; there were pertinent differences in the presence or absence of potential biomarker peaks to differentiate isolates. We also evaluated this method for potential discrimination between different carbapenemases bacteria, utilizing the same strategy. Based on our data and pending further investigation in other CREs, MALDI-TOF MS has potential as a diagnostic tool for the rapid identification of even closely related carbapenemases but would require a paradigm shift in which Biotyper suppliers enable more flexible software control of mass spectral profiling methods.
机译：直接质谱分析的方法对于快速鉴定细菌和真菌的临床分离株是可靠且可重现的。这是第一项评估MALDI-TOF质谱分析技术用于快速鉴定耐碳青霉烯酶的肠杆菌科（CRE）的方法的研究。通过使用基于蛋白质的MALDI Biotyper MS鉴别CRE，可以实现概念验证。通过肉眼观察一致的独特峰（尽管强度很低），似乎可以从平均光谱（MSP）方法中获得该轮廓，因此很有希望。需要优化Biotyper MSP的创建和鉴定方法，以显着改善分数差异，以便在有或没有碳青霉烯酶的情况下进行亚种鉴定。在所有情况下，对这些光谱进行目测峰拾取;在区分分离株的潜在生物标记峰存在与否方面存在相关差异。我们还使用相同的策略评估了这种方法对不同碳青霉烯酶之间的潜在区分。根据我们的数据并有待其他CRE进行进一步研究，MALDI-TOF MS有潜力作为快速识别甚至密切相关的碳青霉烯酶的诊断工具，但需要进行范式转变，Biotyper供应商必须能够更灵活地控制质谱图谱的软件控制方法。

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