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首页> 外文期刊>Frontiers in Veterinary Science >Failure to remove bluetongue serotype 8 virus (BTV-8) from in vitro produced and in vivo derived bovine embryos and subsequent transmission of BTV-8 to recipient cows after embryo transfer.
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Failure to remove bluetongue serotype 8 virus (BTV-8) from in vitro produced and in vivo derived bovine embryos and subsequent transmission of BTV-8 to recipient cows after embryo transfer.

机译：无法从体外产生的和体内衍生的牛胚胎中去除蓝舌血清型8病毒（BTV-8），并且在胚胎移植后将BTV-8传播至受体牛。

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摘要

The behaviour of BTV-8 in cattle is different from most other serotypes not only with regards to clinical signs but certainly with respect to virus transmission (transplacental, contact). Therefore, the possibility of virus transmission by means of embryo transfer was examined by in vitro exposure of in vitro produced and in vivo derived bovine blastocysts to BTV-8 followed by different washing protocols, including longer exposure times (up to 120 s) to 0.25% trypsin at room temperature or at 37°C. None of the washing protocols used was successful in removing the viral genome completely from the in vitro produced and in vivo derived embryos as was demonstrated by real-time PCR. Moreover BTV-8 virus was transmitted to recipient cows after embryo transfer of in vivo derived BTV8-exposed embryos, which had been subjected to routine decontamination as recommended by IETS, consisting of 5 washes in PBS followed by a double treatment of 0.25 % trypsin for 45s at 37°C, and an additional 5 washes in PBS with 2% FCS. This study clearly demonstrates the necessity of vigorous application of the directives for screening of potential donors and the collected embryos, especially in regions with BTV-8, to prevent transmission of the disease.
机译：BTV-8在牛中的行为不仅在临床症状方面而且在病毒传播（胎盘，接触）方面与大多数其他血清型不同。因此，通过将体外产生的和体内衍生的牛胚泡体外暴露于BTV-8，然后采用不同的洗涤方案，包括更长的暴露时间（长达120 s）至0.25，来检查通过胚胎转移传播病毒的可能性。 ％胰蛋白酶在室温或37°C下。如实时PCR所证实，所使用的洗涤方案均不能成功地从体外产生的和体内衍生的胚胎中完全除去病毒基因组。此外，BTV-8病毒是在体内转移了BTV8的胚胎的胚胎转移后传播给受体牛的，这些胚胎已按照IETS的建议进行了常规净化处理，包括在PBS中洗涤5次，然后对0.25％胰蛋白酶进行两次处理。在37°C的温度下搅拌45秒，然后在含2％FCS的PBS中另外洗涤5次。这项研究清楚地表明，有力地应用该指令筛选潜在的供体和收集到的胚胎的必要性，特别是在具有BTV-8的地区，以防止该疾病的传播。

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