...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Medical science monitor : >The effect of short-term administration of (-)-deprenyl and isatin on the expressions of some genes in the mouse brain cortex
【24h】

The effect of short-term administration of (-)-deprenyl and isatin on the expressions of some genes in the mouse brain cortex

机译：短期施用（-）-异戊二烯基和isatin对小鼠大脑皮层某些基因表达的影响

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background Isatin (indoledione 2,3) is an endogenous indole found in the mammalian brain, peripheral tissues, and body fluids. It exhibits many neurophysiological and neuropharmacological effects. It shares some common molecular targets with (-)-deprenyl, a neuroprotective pharmacological drug. Some isatin effects imply a possible influence of gene expression; however, no isatin-responsive genes have yet been identified. Material and Method In this study the effects of a three-week administration of isatin (20 mg/kg) or (-)-deprenyl (1 mg/kg) on the expressions of several putative isatin/deprenyl-responsive genes in the mouse cortex were compared using real-time PCR. Results Both treatments caused similarly significant decreases in superoxide dismutase (SOD) mRNA. Treatment of mice with either drug decreased glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA, although only in the deprenyl-treated mice was this significant (p<0.01). No significant changes were found in cortex mRNA content of monoamine oxidase A or monoamine oxidase B. Conclusions The results suggest that isatin and (-)-deprenyl have some common target genes and this supports the idea that isatin may be an endogenous partial functional agonist of (-)-deprenyl. Since GAPDH mRNA expression is sensitive to the pharmacological treatments, these results also question the applicability of GAPDH as a reference gene in gene expression studies.
机译：背景技术Isatin（吲哚二酮2,3）是在哺乳动物的大脑，外周组织和体液中发现的内源性吲哚。它表现出许多神经生理和神经药理作用。它与神经保护药理药物（-）-异戊烯基共有一些共同的分子靶标。某些isatin效应暗示了基因表达的可能影响;但是，尚未鉴定出对isatin有反应的基因。材料和方法在这项研究中，三周服用isatin（20 mg / kg）或（-）-deprenyl（1 mg / kg）对小鼠皮质中几种假定的isatin / deprenyl反应基因表达的影响使用实时PCR进行比较。结果两种处理均引起超氧化物歧化酶（SOD）mRNA的相似显着降低。尽管仅在经异戊二烯基处理的小鼠中，丙二醛-3-磷酸甘油脱氢酶（GAPDH）mRNA的表达显着降低（p <0.01），但任一种药物均能降低其表达。单胺氧化酶A或单胺氧化酶B的皮质mRNA含量未发现显着变化。结论结果表明，isatin和（-）-deprenyl具有某些共同的靶基因，这支持isatin可能是Isatin的内源性部分功能激动剂的想法。 （-）-异戊二烯基。由于GAPDH mRNA表达对药物治疗敏感，因此这些结果也质疑了GAPDH作为参考基因在基因表达研究中的适用性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号