...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Medical science monitor : >Immunohistochemical analysis of the interstitial mast cells in acute rejection of human renal allografts
【24h】

Immunohistochemical analysis of the interstitial mast cells in acute rejection of human renal allografts

机译：人肾同种异体移植急性排斥反应中间质肥大细胞的免疫组织化学分析

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background:Morphometric investigations were performed to determine whether mast cells correlate with tubulointerstitial fibrosis in acute renal transplant rejection (ARTR) and to examine the relationship between mast cells, interstitial a-smooth muscle actin (α-SMA) expression, and interstitial infiltrates.Material/Methods: Twenty-four renal allograft biopsy specimens from patients with ARTR were examined quantitatively by means of a computer image analysis system. As a control 11 allograft biopsy specimens were used from patients with no signs of rejection.Results: The morphometric study revealed that the mean values of the interstitial tryptase positive cells, expression of α-SMA, interstitial volume, CD 68+, CD 43+ and CD 20+ cells were significantly increased in ARTR patients in comparison to controls. In the ARTR group there were significant positive correlations between interstitial tryptase positive cells and interstitial expression of α-SMA, interstitial volume, CD 43+ and CD 68+. Moreover, CD 68 positive cells significantly correlated with interstitial volume (r=0.51, p0.02). The correlations between interstitial tryptase positive cells and CD 20+ cells as well as between CD 43+ and CD 20+ cells and interstitial volume were positive, but did not reach statistical significance.Conclusions: Our findings demonstrate that mast cells are one of the constitutive cell types in the interstitium in ARTR. Although the significant positive correlations between interstitial mast cell count and relative interstitial volume, as well as interstitial expression of a-SMA, suggest that these cells have a role in the development of early interstitial fibrosis in renal allografts, these relationships need further investigation.
机译：背景：进行形态计量学检查以确定肥大细胞是否与急性肾移植排斥反应（ARTR）中的肾小管间质纤维化相关，并检查肥大细胞，间质a-平滑肌肌动蛋白（α-SMA）表达与间质浸润之间的关系。方法：采用计算机图像分析系统对来自ARTR患者的24例肾脏同种异体移植活检标本进行了定量检查。作为对照，使用11例无排斥迹象的同种异体活检标本。结果：形态计量学研究显示间质类胰蛋白酶阳性细胞的平均值，α-SMA的表达，间质体积，CD 68 +，CD 43+与对照组相比，ARTR患者的CD20 +和CD 20+细胞显着增加。在ARTR组中，间质类胰蛋白酶阳性细胞与间质α-SMA表达，间质体积，CD 43+和CD 68+之间存在显着的正相关。而且，CD 68阳性细胞与间质体积显着相关（r ＝ 0.51，p ＜0.02）。间质类胰蛋白酶阳性细胞和CD 20+细胞之间以及CD 43+和CD 20+细胞之间的相关性与间质体积呈正相关，但没有统计学意义。结论：我们的研究结果表明肥大细胞是组成型之一ARTR间质中的细胞类型。尽管间质肥大细胞计数与相对间质体积以及a-SMA的间质表达之间存在显着的正相关关系，表明这些细胞在同种异体肾移植早期间质纤维化的发展中具有一定作用，但这些关系仍需进一步研究。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号